Utilization potential of agro-industrial by-products and waste sources: Laccase production in bioreactor with Pichia pastoris

Article Environmental Sciences

Magnetized manganese-doped watermelon rind biochar as a novel low-cost catalyst for improving oxygen reduction reaction in microbial fuel cells

Jiwei Jiang et al.

Summary: A novel cathode catalyst was developed using watermelon rind as raw material, which significantly enhanced the electrochemical performance of MFCs, reduced costs, and achieved significant effects in water treatment and power generation.

SCIENCE OF THE TOTAL ENVIRONMENT (2022)

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Article Environmental Sciences

High Production of Trametes cinnabarina Laccase (lac1) by Suspended and Immobilized Cells of Recombinant Pichia pastoris from Crude Glycerol

Aniket Bhattacharyya et al.

Summary: In this study, a cost-effective and efficient process for high-level fungal laccase production from crude glycerol was developed. The laccase gene was chemically synthesized and integrated into Pichia pastoris strains to facilitate constitutive secretory expression of laccase. The recombinant strains showed high levels of laccase production from pure glycerol and crude glycerol media. Additionally, immobilization of the cells using different matrices improved laccase production and stability.

WASTE AND BIOMASS VALORIZATION (2022)

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Article Environmental Sciences

Valorisation of watermelon (Citrullus lanatus) rind waste into bioethanol: an optimization and kinetic studies

M. A. Kassim et al.

Summary: The study examined the impact of fermentation operating parameters on bioethanol production from watermelon rind waste. It was found that high bioethanol yields could be achieved under specific conditions. Using mathematical models, the growth kinetics of yeast during fermentation process were successfully described. The results provide valuable information for further development of bioethanol production processes.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (2022)

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Article Food Science & Technology

Improved enzyme production on corncob hydrolysate by a xylose-evolved Pichia pastoris cell factory

Olufemi Emmanuel Bankefa et al.

Summary: The study demonstrated the potential of lignocellulosic biomass in biomanufacturing by producing enzymes from corn cob hydrolysate. By selecting appropriate mutants, the utilization efficiency of xylose was successfully optimized. The results showed that this approach could effectively improve enzyme production.

JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE (2022)

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Article Agricultural Engineering

Fungal pretreatments of Napier grass and sugarcane leaves for high recovery of lignocellulosic enzymes and methane production

Rawitsara Intasit et al.

Summary: This study explores the potential of Napier grass and sugarcane for lignocellulosic enzyme and biomethane production through biological pretreatment. It finds that fungal pretreatment can produce highly active xylanase and cellulase, while yeast pretreatment has higher cellulase and xylanase productivity. Additionally, a mixture of Xylaria-pretreated Napier grass and cow manure can increase biogas yield and methane content.

INDUSTRIAL CROPS AND PRODUCTS (2022)

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Article Green & Sustainable Science & Technology

Agricultural waste biorefinery development towards circular bioeconomy

Mukesh Kumar Awasthi et al.

Summary: The biorefinery concept relies on the recovery of high-value chemicals, and this study critically reviewed agricultural waste management technologies to explore the potential for biorefinery in waste management and its development opportunities.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2022)

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Article Energy & Fuels

Watermelon peel hydrolysate production optimization and ethanologenesis employing yeast isolates

Asma Chaudhary et al.

Summary: Securing energy from waste is a major research area for finding global solutions for waste disposal and energy production. This study investigates the potential of using discarded watermelon peels as a source for ethanol production through fermentation using yeast. The optimized parameters for fermentation were determined using response surface methodology, and it was found that the yeast strain Metchnikowia cibodasensis Y34 showed significant potential for valorizing watermelon peels by producing ethanol.

BIOMASS CONVERSION AND BIOREFINERY (2022)

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Review Biotechnology & Applied Microbiology

Second generation Pichia pastoris strain and bioprocess designs

Burcu Gunduz Ergun et al.

Summary: This review evaluates the potential of P. pastoris for circular bioeconomy and discusses challenges and future perspectives for second-generation platforms. The use of low-cost and renewable substrates, as well as the development of metabolic engineering pathways, are explored. The importance of second-generation bioprocess development for waste recycling and the environmental advantages of P. pastoris are also highlighted.

BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS (2022)

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Article Biochemistry & Molecular Biology

Optimisation of the Production and Bleaching Process for a New Laccase from Madurella mycetomatis, Expressed in Pichia pastoris: from Secretion to Yielding Prominent

Ahmet Tulek et al.

Summary: Laccases, particularly the cloned Madurella mycetomatis laccase expressed in Pichia pastoris, showed optimal biochemical conditions and structural properties, making it suitable for applications in decolourisation and textile bleaching. The enzyme MmLac demonstrated good activity and stability in acidic pH range, as well as under various environmental conditions, highlighting its potential for textile biotechnology and other industrial applications.

MOLECULAR BIOTECHNOLOGY (2021)

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Article Biochemical Research Methods

Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris

Miao Tian et al.

Summary: Utilizing crude glycerol as a carbon source improves the efficiency of lipase production by Pichia pastoris, with impurities such as Na+, Ca2+, and grease playing significant roles in enhancing lipase activity. Optimization of Ca2+, Na+, and grease concentrations can significantly increase lipase activity, highlighting the economical and efficient valorization of crude glycerol.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2021)

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Article Green & Sustainable Science & Technology

An innovative strategy of recycling miscellaneous waste carbohydrates from high-fructose syrup production for Pichia pastoris fermentation

Minjie Gao et al.

Summary: Utilizing waste liquid containing miscellaneous waste carbohydrates (MWC) from the high-fructose syrup (HFS) production industry in China as an alternative carbon source for Pichia pastoris fermentation, the study successfully optimized the fermentation processes and achieved high production of endo-beta-1,3-glucanase. This cleaner production approach significantly reduced environmental pollution and production costs in both HFS and P. pastoris fermentation industries.

JOURNAL OF CLEANER PRODUCTION (2021)

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Article Biotechnology & Applied Microbiology

Characterization of a recombinant laccase from Fusarium oxysporum HUIB02 for biochemical application on dyes removal

Nguyen Duc Huy et al.

Summary: In this study, a putative laccase gene was cloned from Fusarium oxysporum HUIB02 and expressed in P. pastoris. The recombinant laccase rFoLacc5 exhibited great potential for biotechnological applications in synthetic dyes removal. Additionally, the optimal culture conditions for enzyme production were optimized to enhance the enzyme activity.

BIOCHEMICAL ENGINEERING JOURNAL (2021)

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Article Engineering, Chemical

Optimization of a Recombinant Lectin Production in Pichia pastoris Using Crude Glycerol in a Fed-Batch System

Dulce Palmerin-Carreno et al.

Summary: The study focuses on producing recombinant lectin using engineered Pichia pastoris yeast, optimizing the process by using crude glycerol from biodiesel production. The results demonstrate that recombinant lectin can be efficiently produced from crude glycerol, showing the potential for high productivity in producing heterologous proteins.

PROCESSES (2021)

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Article Food Science & Technology

Modelling the Extraction of Pectin towards the Valorisation of Watermelon Rind Waste

Daniel Alexander Mendez et al.

Summary: This study utilized an experimental design to investigate the impact of extraction parameters on watermelon rind pectin, resulting in the discovery of novel compositional features and the potential to produce customized pectin ingredients with wider application scope. Optimum conditions yielded WRP with comparable purity and molar mass but higher branching degree compared to traditional pectin sources. Different extraction conditions generated pectin structures with varying characteristics.

FOODS (2021)

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Article Biology

Recombinant laccase production from Bacillus licheniformis O12: Characterization and its application for dye decolorization

Arzu Ozturk Kesebir et al.

Summary: This study successfully produced a novel, highly thermostable laccase from Bacillus licheniformis O12 bacterial DNA using a recombinant method and investigated its decolorization effects on some dyes found in textile wastewaters. The obtained laccase was highly resistant to pH and temperature, showing promising potential for practical applications in wastewater treatment.

BIOLOGIA (2021)

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Article Biotechnology & Applied Microbiology

Development of wheat bran hydrolysate as Komagataella phaffii medium for heterologous protein production

Ziwei Zhou et al.

Summary: The optimal detoxification process of wheat bran hydrolysate (WBH) was established in this research for the expression of heterologous protein by Komagataella phaffii, resulting in a low-cost medium alternative and a promising outlet for wheat bran utilization.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2021)

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Review Biotechnology & Applied Microbiology

Recombinant protein production in Pichia pastoris: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling

Burcu Gundus Ergun et al.

Summary: Pichia pastoris is widely used for recombinant protein production. Research has focused on improving control of gene expression and extending the production window, as well as developing more efficient protein production platforms through engineering promoters and transcription factors.

FEMS YEAST RESEARCH (2021)

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Review Biotechnology & Applied Microbiology

What makes Komagataella phaffii non-conventional?

Oezge Ata et al.

Summary: K. phaffii, classified as a non-conventional yeast, stands out from both conventional yeast like Saccharomyces cerevisiae and other non-conventional yeasts in terms of regulatory features, transcriptional networks, and Golgi apparatus structure. Its distinct characteristics make it a promising subject for both fundamental research and industrial applications.

FEMS YEAST RESEARCH (2021)

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Review Food Science & Technology

Principles, drivers and opportunities of a circular bioeconomy

Abigail Muscat et al.

Summary: This study proposes five ecological principles for guiding biomass use towards a circular bioeconomy. Implementing these principles calls for fundamental changes to policies, technologies, organizations, social behavior, and markets.

NATURE FOOD (2021)

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Review Biology