期刊
AUTOPHAGY
卷 19, 期 10, 页码 2817-2818出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2023.2182595
关键词
Autophagy; Drosophila; germline; mitochondria; mitochondrial DNA; mitophagy; mtDNA; purifying selection
类别
Female germline has evolved a programmed germline mitophagy (PGM) to control the quality of mitochondrial DNA (mtDNA). PGM is induced by the inhibition of mTorC1 and requires the autophagy machinery and the mitophagy adaptor BNIP3, but not Pink1 and park, for germline mtDNA quality control. The study also identified Atx2 as a major regulator of PGM.
Mitochondrial DNA (mtDNA) is prone to the accumulation of mutations. To prevent harmful mtDNA mutations from being passed on to the next generation, the female germline, through which mtDNA is exclusively inherited, has evolved extensive mtDNA quality control. To dissect the molecular underpinnings of this process, we recently performed a large RNAi screen in Drosophila and uncovered a programmed germline mitophagy (PGM) that is essential for mtDNA quality control. We found that PGM begins as germ cells enter meiosis, induced, at least in part, by the inhibition of the mTor (mechanistic Target of rapamycin) complex 1 (mTorC1). Interestingly, PGM requires the general macroautophagy/autophagy machinery and the mitophagy adaptor BNIP3, but not the canonical mitophagy genes Pink1 and park (parkin), even though they are critical for germline mtDNA quality control. We also identified the RNA-binding protein Atx2 as a major regulator of PGM. This work is the first to identify and implicate a programmed mitophagy event in germline mtDNA quality control, and it highlights the utility of the Drosophila ovary for studying developmentally regulated mitophagy and autophagy in vivo.
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