Complete genome sequence of a novel lytic phage of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight pathogen in rice

Bacteriophage L522, which infects Xanthomonas oryzae pv. oryzae, was isolated from a paddy leaf sample collected in Long An province, Vietnam. The phage shows myovirus morphology based on transmission electron microscopy. It displays a latent period and burst size of approximately 3 h and 63 new virions per infected cell (PFU/infected cell), respectively. The genome of L522 is 44,497 bp in length, with 52% GC content. Of the 63 genes identified, functions were predicted for 26. No virulence or antibiotic-resistance genes were detected. The results of a BLASTn search showed similarity to a previously reported Xanthomonas phage, with 85% average nucleotide sequence identity and 87.15% query coverage. Thus, this L522 is a representative of a new species in the genus Xipdecavirus.

Automated summary

Bacteriophage L522 was isolated from a paddy leaf sample in Long An province, Vietnam, and infects Xanthomonas oryzae pv. oryzae. Transmission electron microscopy revealed its myovirus morphology. It has a latency period of approximately 3 hours and a burst size of 63 new virions per infected cell (PFU/infected cell). The genome of L522 is 44,497 bp long with a GC content of 52%. Out of the 63 identified genes, 26 were predicted to have functions, and no virulence or antibiotic-resistance genes were detected. A BLASTn search revealed similarity to a previously reported Xanthomonas phage, with an average nucleotide sequence identity of 85% and a query coverage of 87.15%. Thus, L522 represents a new species in the genus Xipdecavirus.