Complete genome sequence of cnidium virus 2, a novel cytorhabdovirus isolated from Cnidium officinale in South Korea

High-throughput sequencing identified a cytorhabdovirus, tentatively named cnidium virus 2 (CnV2), in Cnidium officinale, and Sanger sequencing confirmed the genome sequence. CnV2 is 13,527 nucleotides in length and contains seven open reading frames in the order 3 '-N-P-3-4-M-G-L-5 ', separated by intergenic regions. The full-length nucleotide sequence of CnV2 shares 19.4-53.8% identity with other known cytorhabdovirus genome sequences. The N, P, P3, M, G, and L proteins share 15.8-66.7%, 11-64.3%, 11.1-80.5%, 10.8-75.3%, 12.3-72.1%, and 20-72.7% amino acid sequence identity, respectively, with the cognate deduced protein sequences from known cytorhabdoviruses. CnV2 is related to other members of the genus Cytorhabdovirus, with sambucus virus 1 being the closest relative. Thus, CnV2 should be classified as a new member in the genus Cytorhabdovirus of the family Rhabdoviridae.

Automated summary

A new cytorhabdovirus named cnidium virus 2 (CnV2) was identified in Cnidium officinale through high-throughput sequencing. The genome sequence of CnV2 was confirmed by Sanger sequencing. CnV2 belongs to the genus Cytorhabdovirus of the family Rhabdoviridae and shares sequence similarities with other known cytorhabdoviruses.