Characteristics of tissue distribution, metabolism, effects on brain catecholamines, and environmental exposure of frogs to neonicotinoid insecticides

Pesticide exposure is considered to be one important factor responsible for declining amphibian populations worldwide. The usage of neonicotinoid insecticides (NNIs) has markedly increased in recent years, and there are concerns regarding the effects of NNI-induced toxicity on the development and behavior of amphibians. How-ever, there have been few reports on the metabolism, distribution, and neurotoxicity of NNIs in amphibians. In this study, we exposed the Western clawed frog (Silurana tropicalis) to clothianidin (CLT) in water. After 24 h of exposure, the highest concentrations were detected in the skin, indicating that frogs are at a high risk of absorbing CLT through their skin along with water. Excretion of CLT was estimated based on the concentrations of CLT metabolites in the water until 48 h of exposure. The findings showed that frogs had higher CLT metabolic ability than zebrafish. Serotonin levels in the brain were lower in the high-concentration CLT exposure group than in the control group, although the difference was not statistically significant. This suggested that catecholamine-related effects of CLT on the brain cannot be disregarded. In addition, quantitative analyses of NNI residue in wild frogs, soil, and water in agricultural areas in Hokkaido, Japan, were performed and four NNIs were detected. These results indicated the possible risk of NNI-induced toxicity in frogs. This is the first report of the characteristics of tissue distribution and metabolism of NNIs in frogs, which may facilitate the design of appropriate conservation programs for amphibians.

Automated summary

Pesticide exposure, especially neonicotinoid insecticides (NNIs), is a major factor contributing to the decline of amphibian populations worldwide. However, the metabolism, distribution, and neurotoxicity of NNIs in amphibians remain poorly understood. This study exposed Western clawed frogs to clothianidin (CLT) and found that the highest concentrations of CLT were detected in the skin, indicating high risk of skin absorption. Frogs also showed higher CLT metabolic ability compared to zebrafish. Additionally, NNI residues were detected in wild frogs, soil, and water in agricultural areas of Japan, suggesting the potential toxicity of NNIs to amphibians.