Characterization and evaluation of substratum material selection for microalgal biofilm cultivation

Biofilm cultivation is considered a promising method to achieve higher microalgae biomass productivity with less water consumption and easier harvest compared to conventional suspended cultivation. However, studies focusing on the selection of substratum material and optimization of the growth of certain microalgae species on specific substratum are limited. This study investigated the selection of membranous and fabric fiber substrata for the attachment of unicellular microalgae Scenedesmus dimorphus and filamentous microalgae Tribonema minus in biofilm cultivation. The results indicated that both algal species preferred hydrophilic membranous substrata and nitrate cellulose/cellulose acetate membrane (CN-CA) was selected as a suitable candidate on which the obtained biomass yields were up to 10.24 and 7.81 g m(-2) day(-1) for S. dimorphus and T. minus, respectively. Furthermore, high-thread cotton fiber (HCF) and low-thread polyester fiber (LPEF) were verified as the potential fabric fiber substrata for S. dimorphus (5.42 g m(-2) day(-1)) and T. minus (5.49 g m(-2) day(-1)) attachment, respectively. The regrowth of microalgae biofilm cultivation strategy was applied to optimize the algae growth on the fabric fiber substrata, with higher biomass density and shear resistibility achieved for both algal species. The present data highlight the importance to establish the standards for selection the suitable substratum materials in ensuring the high efficiency and sustainability of the attached microalgal biomass production.

Automated summary

Biofilm cultivation is a promising method for achieving higher microalgae biomass productivity. This study investigated the selection of substratum material for the attachment of two microalgae species. It was found that hydrophilic membranous substrata and specific types of fabric fiber substrata were preferred by the microalgae, resulting in high biomass yields. Optimal growth conditions were also identified for both species using a regrowth strategy. The findings highlight the importance of selecting suitable substratum materials for efficient and sustainable microalgal biomass production.