Influence of Borassus flabellifer Endocarps Hydrolysate on Fungal Biomass and Fatty Acids Production by the Marine Fungus Aspergillus sp.

Polyunsaturated Fatty Acids (PUFAs) are important nutrients for human health. We aimed to evaluate the efficiency of marine water fungus Aspergillus sp. (Accession no: MZ505709) for lipid biosynthesis. The Yeast Extract Glucose (YEG) medium was supplemented with different concentration of Borassus flabellifer Endocarps Hydrolysate (BFEH; 1-5%) to evaluate the fungal biomass and its lipid accumulation. The combination of glucose and BFEH as carbon source increased the fresh weight (25.43 +/- 0.33 g/L), dry weight (21.39 +/- 0.77 g/L) and lipid yield (3.14 +/- 0.09 g/L) of fungal biomass. The lipid content of dried fungal biomass has shown 91.08 +/- 5.07 mg cod liver oil equivalents/g and 125.98 +/- 5.96 mg groundnut oil equivalents/g biomass. GC-MS and NMR spectrometry analysis revealed the compounds involved in fatty acid metabolism and lipid signaling pathways along with the presence of linolenic acid. Interestingly, fungus grown in BFEH enriched medium has recorded the maximum amount of lipids with major fatty acid derivatives. Increase in the growth rate of Artemia franciscana was observed, when the extracted fungal lipid was supplemented as a food supplement. Therefore, this study suggests that marine fungal lipid may serve as potential natural compound as nutraceuticals and aquafeeds.

Automated summary

The marine water fungus Aspergillus sp. (Accession no: MZ505709) shows high efficiency in lipid biosynthesis, especially when supplemented with Borassus flabellifer Endocarps Hydrolysate (BFEH). The combination of glucose and BFEH as carbon source enhances the fungal biomass and its lipid accumulation.