In Silico Analysis and Immune Response of YaeT Protein Against Riemerella anatipestifer in Ducks

Riemerella anatipestifer (RA) is one of the most harmful bacterial pathogens in waterfowl and causes enormous economic loss worldwide. Due to weak cross-immunity protection against different serotypes of RA, inactivated and attenuated vaccines are only effective for RA of specific serotypes. In this paper, outer membrane protein YaeT in RA was analyzed through bioinformatics, in vivo, and in vitro assays. Homology, physicochemical and structural properties, transmembrane domains, and B-cell binding epitopes were investigated. The recombinant outer membrane protein YaeT was then inoculated into Cherry Valley ducks to analyze its immune protection against RA. Results showed that the protein was conservative in different RA strains and had sufficient B-cell binding epitopes. The immunized duck serum contains high-affinity antibodies that could activate complement and promote the opsonophagocytosis of RA by phagocytes. After RA challenge, the survival rate of the YaeT protein-immunized ducks was 80%.

Automated summary

In this study, the outer membrane protein YaeT in RA was analyzed, and it was found to be conservative and have sufficient B-cell binding epitopes. Immunization with YaeT protein led to the production of high-affinity antibodies in duck serum, which could activate complement and promote the attack of phagocytes against RA. After RA challenge, the survival rate of YaeT protein-immunized ducks reached 80%.