Lipid Accumulation by Snf-β Engineered Mucor circinelloides Strains on Glucose and Xylose

Sucrose non-fermenting 1 (SNF1) protein kinase plays the regulatory roles in the utilization of selective carbon sources and lipid metabolism. Previously, the role of ss subunit of SNF1 in lipid accumulation was evaluated by overexpression and knockout of Snf-ss in oleaginous fungus M. circinelloides. In the present study, the growth and lipid accumulation of Snf-ss overexpression and knockout strains were further analyzed and compared with glucose or xylose as a single or mixed carbon sources. The results showed that the lipid contents in Snf-ss knockout strain improved by 23.2% (for glucose), 28.4% (for xylose), and 30.5% (for mixed glucose and xylose) compared with that of the control strain, respectively. The deletion of Snf-ss subunit also altered the transcriptional level of acetyl-CoA carboxylase (ACC). The highest transcriptional levels of ACC1 in Snf-ss knockout strain at 24 h were increased by 2.4-fold (for glucose), 2.8-fold (for xylose), and 3.1-fold (for mixed glucose and xylose) compared with that of the control strain, respectively. Our results indicated that Snf-ss subunit enhanced lipid accumulation through the regulation of ACC1 in response to xylose or mixed sugars of glucose and xylose more significantly than that of response to glucose. This is the first study to explore the effect of Snf-ss subunit of M. circinelloides in regulating lipid accumulation responding to different carbon nutrient signals of glucose and xylose. This study provides a foundation for the future application of the Snf-ss engineered strains in lipid production from lignocellulose.

Automated summary

In this study, the effects of Snf-ss subunit on the growth and lipid accumulation of M. circinelloides were analyzed and compared under different carbon nutrient signals. The results showed that the knockout of Snf-ss subunit resulted in significantly increased lipid accumulation, especially in response to xylose or mixed glucose and xylose. Furthermore, the transcriptional level of ACC1 was also affected by the deletion of Snf-ss subunit. This study provides insights into the regulation of lipid production in M. circinelloides and its potential application in lignocellulosic lipid production.