4.4 Article

Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Lawsonia inermis Against Common Pathogens from Urinary Tract Infection

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SPRINGER
DOI: 10.1007/s12010-023-04482-1

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Silver nanoparticles; Lawsonia inermis Linn; Antibacterial activity; Urinary tract infection

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This study focuses on the biological approach of silver nanoparticles (Ag-NPs) using Egyptian henna leaves extracts and analysis of the prepared Ag-NPs. The components of the plant extracts were identified using gas chromatography mass spectrometry (GC-mass). The prepared Ag-NPs were characterized using UV-visible (UV-Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and Fourier transform infrared (FTIR) analysis. The antibacterial activities of the Ag-NPs were examined and found to be effective against various microorganisms.
New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range of consumer items, are of significant interest. Hence, this research emphasizes the biological approach of Ag-NPs through Egyptian henna leaves (Lawsonia inermis Linn.) extracts and analysis of the prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses of prepared Ag-NPs were carried out through UV-visible (UV-Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and Fourier transform infrared (FTIR) analysis. UV-Vis reveals that Ag-NPs have a maximum peak at 460 nm in visible light. Structural characterization recorded peaks that corresponded to Bragg's diffractions for silver nano-crystal, with average crystallite sizes varying from 28 to 60 nm. Antibacterial activities of Ag-NPs were examined, and it is observed that all microorganisms are very sensitive to biologically synthesized Ag-NPs.

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