4.3 Article

Inhibition of mitochondrial VDAC1 oligomerization alleviates apoptosis and necroptosis of retinal neurons following OGD/R injury

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ELSEVIER GMBH
DOI: 10.1016/j.aanat.2023.152049

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VDAC1 oligomerization; OGD; R; AHIOP; Mitochondrial dysfunction; Apoptosis; Necrosis

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Ischemia-reperfusion (I/R) injury is a common pathological mechanism in retinal diseases, and mitochondrial dysfunction plays a critical role in cell death. Voltage-dependent anion channel 1 (VDAC1) regulates the permeability of mitochondrial membrane by controlling its oligomerization. This study demonstrates that inhibition of VDAC1 oligomerization can rescue mitochondrial dysfunction and reduce cell death in retinal I/R injury, suggesting a potential therapeutic strategy for ocular diseases caused by I/R injury.
Ischemia-reperfusion (I/R) injury is a common pathological mechanism in many retinal diseases, which can lead to cell death via mitochondrial dysfunction. Voltage-dependent anion channel 1 (VDAC1), which is mainly located in the outer mitochondrial membrane, is the gatekeeper of mitochondria. The permeability of mitochondrial membrane can be regulated by controlling the oligomerization of VDAC1. However, the functional mechanism of VDAC1 in retinal I/R injury was unclear. Our results demonstrate that oxygen-glucose deprivation and re-oxygenation (OGD/R) injury leads to apoptosis, necroptosis, and mitochondrial dysfunction of R28 cells. The OGD/R injury increases the levels of VDAC1 oligomerization. Inhibition of VDAC1 oligomerization by VBIT-12 rescued mitochondrial dysfunction by OGD/R and also reduced apop-tosis/necroptosis of R28 cells. In vivo, the use of VBIT-12 significantly reduced aHIOP-induced neuronal death (apoptosis/necroptosis) in the rat retina. Our findings indicate that VDAC1 oligomers may open and enlarge mitochondrial membrane pores during OGD/R injury, leading to the release of death-related factors in mitochondria, resulting in apoptosis and necroptosis. This study provides a potential therapeutic strategy against ocular diseases caused by I/R injury. (c) 2023 Elsevier GmbH. All rights reserved.

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