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Letter
Infectious Diseases
Prerna Arora et al.
LANCET INFECTIOUS DISEASES
(2023)
Article
Cell Biology
Yanqun Wang et al.
Summary: Researchers isolated two neutralizing antibodies BA7208 and BA7125 from mice engineered to produce human antibodies. BA7125 showed broad neutralizing activity against all variants, while BA7208 was highly potent neutralizing against all tested SARS-CoV-2 variants except Mu. By combining BA7208 and BA7125, a biparatopic antibody BA7208/7125 that neutralized all tested variants was generated. Cryo-electron microscopy structure analysis showed that the contact residues of these antibodies were conserved and had minimal interactions with mutational residues in RBD of current variants. In addition, BA7208/7125 demonstrated potent therapeutic efficacy against Omicron BA.1 and BA.2 in mice and could be used for effective prophylaxis.
Article
Biochemistry & Molecular Biology
Jie Yang et al.
Summary: In this study, we successfully engineered the Leptotrichia wadei (Lwa) Cas13a protein by inserting different RNA-binding domains, resulting in enhanced collateral activity and improved sensitivity. By combining with an electrochemical method, our engineered variants showed the capability to detect the SARS-CoV-2 genome at extremely low concentrations without target preamplification. This study provides a highly sensitive approach for nucleic acid detection.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Analytical
Ying Deng et al.
Summary: In this study, a sensitive and accurate electrochemical method was designed to detect the SARS-CoV-2 virus using two target genes. The method successfully detected the virus in the range of 0.1-5000 pM with a detection limit of 57 fM. It also distinguished COVID-19 samples from healthy individuals and showed good agreement with RT-PCR results, demonstrating its potential for clinical application.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Panke Qu et al.
Summary: The emergence of new Omicron subvariants, including BQ.1, BQ.1.1, BA.4.6, BF.7, and BA.2.75.2, has shown enhanced neutralization resistance against sera from vaccinated healthcare workers and COVID-19 patients. The mutations N460K, K444T, and F486S play a significant role in driving the increased neutralization resistance of these subvariants. These findings provide insights into the evolution of SARS-CoV-2 Omicron subvariants.
CELL HOST & MICROBE
(2023)
Article
Chemistry, Multidisciplinary
Jingfang Zhang et al.
Summary: This study reports the development of aptamer-based spherical nucleic acids (SNAs) that can noninvasively and programmably regulate the activity of target proteins using near-infrared (NIR) light. The photoresponsive SNAs can capture target proteins and function as enzyme inhibitors when reverted by NIR light, and their inhibition functions can be further reversed by antidote DNA.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Hyojin Lee et al.
Summary: This study developed a new method for sensitive detection of SARS-CoV-2 viral RNA without thermal cycling. By using tandem isothermal gene amplification technology, this method can detect very low concentrations of viral RNA within 1 hour, and it has high sensitivity and accuracy for COVID-19 diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Huijie Li et al.
Summary: This study reports a new biosensor for amplification-free nucleic acid detection using Cas13a and ultrasensitive graphene field-effect transistors (gFETs). It achieves the detection of SARS-CoV-2 and RSV genome without target preamplifications, even at very low viral loads, making it one of the most sensitive nucleic acid diagnostic platforms to date.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Medicine, General & Internal
Antonio Vitiello et al.
Summary: This review provides an overview of the molecular profile, epidemiology, transmissibility, impact on vaccines, and pharmacological agents related to the Omicron variant of SARS-CoV-2. Omicron has multiple mutations, including changes in the spike protein, which contribute to its high transmissibility and potential immune escape. Current vaccination provides limited protection against Omicron, while booster doses offer significant protection against mild illness. New oral antiviral agents are emerging as important alternatives for treatment. Further research is needed to determine the infectiousness of Omicron and the effectiveness of available vaccines, monoclonal antibodies, and antiviral drugs.
JOURNAL OF INTERNAL MEDICINE
(2022)
Article
Engineering, Biomedical
Jon Arizti-Sanz et al.
Summary: SARS-CoV-2 and its variants can be visually detected via easy-to-use Cas13-based nucleic acid tests leveraging lyophilised reagents and fast sample inactivation at ambient temperature.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Infectious Diseases
Xiao-Lin Jiang et al.
LANCET INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Yanbing Yang et al.
Summary: A new urinalysis device is reported for the diagnosis, classification, and recurrence assessment of bladder cancer by directly analyzing bladder tumor-associated proteins in urine samples. The device integrates molecular-specificity IGZO FET biosensor arrays, a device control panel, and an internet terminal for remote healthcare and personalized medicine.
ADVANCED MATERIALS
(2022)
Letter
Virology
Perumal A. Desingu et al.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Review
Immunology
Fangxin Zhao et al.
Summary: This review discusses the immune evasion and high variability of SARS-CoV-2 variants, as well as the challenges in designing universal vaccines against these variants. The article summarizes several novel approaches worthy of further study, including construction of chimeric immunogens and design of protein nanoparticle antigens. Some immunogens may induce cross-reactive immune responses against multiple coronaviruses, providing insights for the development of universal vaccines.
Article
Multidisciplinary Sciences
Beatrice Casati et al.
Summary: During the COVID-19 pandemic, the use of PCR testing and antigen tests has been crucial in controlling the spread of SARS-CoV-2. However, these methods have limitations in terms of their applicability and sensitivity. To address this, researchers have developed a diagnostic test called ADESSO, which utilizes Cas13 to accurately diagnose COVID-19 within 1 hour without requiring sophisticated equipment. ADESSO has shown to have comparable sensitivity and specificity to RT-qPCR on extracted RNA and higher performance than antigen tests for unextracted samples. This fast, sensitive, and affordable method can be used in point-of-care settings to diagnose COVID-19 and can easily adapt to detect new variants.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Christia M. Victoriano et al.
Scientific Reports
(2022)
Article
Engineering, Biomedical
Ting Zhang et al.
Summary: This study presents a paper-based assay for the rapid colorimetric detection of SARS-CoV-2 variants. The assay utilizes nucleic acid reactions and enzymatic amplification to detect different variants of the virus within 30 minutes. The results show 100% concordance with other commonly used detection methods. This customizable and inexpensive paper-based assay could facilitate viral surveillance.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Xin Wang et al.
Summary: The development of eco-friendly, ultralow-power and easy-to-process electronics faces challenges in off-the-grid applications like IoTs and extreme environments. Paper-based electronics with E-mode CNT CMOS TFTs show high voltage gains, noise margins, and low power dissipation, making them attractive for energy-saving and environment-friendly ICs in harsh environments.
ADVANCED MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Xiangang Huang et al.
Summary: This Perspective explores the potential role of nanotechnology in addressing the rise of new SARS-CoV-2 variants. Nanotechnology has been instrumental in the fight against SARS-CoV-2, particularly with the development of highly effective nanotechnology-based mRNA vaccines. However, the emergence of new variants has hindered efforts to bring an end to the pandemic, necessitating the proposal of novel strategies to tackle the crisis posed by existing and emerging SARS-CoV-2 variants.
NATURE NANOTECHNOLOGY
(2022)
Editorial Material
Engineering, Biomedical
Ahmed Ghouneimy et al.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Cell Biology
Kirsten E. Lyke et al.
Summary: This study assesses the magnitude and short-term durability of neutralizing antibodies after homologous and heterologous boosting with mRNA and Ad26.COV2.S vaccines. The results show that mRNA vaccine boosting generates higher neutralizing activity against the Omicron variant compared to Ad26.COV2.S boosting. These findings have implications for protecting against Omicron and future variants of SARS-CoV-2.
CELL REPORTS MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Parinaz Fozouni et al.
Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.
Article
Chemistry, Analytical
Chao Zhang et al.
Summary: This study developed a rapid detection method for SARS-CoV-2, with the entire sample-to-result workflow taking less than 40 minutes. The method achieved 100% accuracy compared to PCR-based assays, offering a potential universal platform for rapid and point-of-care detection of emerging infectious diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Wilfredo F. Garcia-Beltran et al.
Summary: New variants of SARS-CoV-2 show high resistance to vaccine neutralization, with some able to escape vaccine responses with just a few mutations, highlighting the importance of developing broadly protective measures against variants.
Review
Microbiology
Jessica A. Plante et al.
Summary: Despite the development of vaccines, COVID-19 caused by SARS-CoV-2 continues to be a global concern due to the emergence of new variants, raising worries about increased spread and potential impacts on immunity.
CELL HOST & MICROBE
(2021)
Article
Chemistry, Multidisciplinary
Yan Hao et al.
Summary: By using a rapid dehydration method, the synthesis of spherical nucleic acids with record-high DNA density is achieved in seconds, resulting in a 3-time increase in DNA density compared to traditional methods. This ultra-high density DNA grafting facilitates the formation of core-satellite assemblies, making the synthesis of spherical nucleic acids easier and enabling future explorations of the physical, chemical, and biological effects of high-density DNA spherical nucleic acids.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Editorial Material
Medicine, General & Internal
Arnaud Fontanet et al.
Article
Multidisciplinary Sciences
Houriiyah Tegally et al.
Summary: The article describes a newly emerged lineage of SARS-CoV-2, 501Y.V2, characterized by eight mutations in the spike protein, which may result in increased transmissibility or immune escape. This lineage originated in South Africa and quickly became dominant in Eastern Cape, Western Cape, and KwaZuluNatal provinces within weeks.
Article
Multidisciplinary Sciences
Nicholas G. Davies et al.
Summary: A new variant of SARS-CoV-2 has emerged in England, with a higher reproduction number and potential for large resurgences of COVID-19 cases. Without stringent control measures, it is projected that COVID-19 hospitalizations and deaths in England for the first 6 months of 2021 will exceed those in 2020. The variant has spread globally and exhibited similar transmission increases in Denmark, Switzerland, and the United States.
Editorial Material
Multidisciplinary Sciences
Daniel M. Altmann et al.
Article
Multidisciplinary Sciences
Thanyarat Chaibun et al.
Summary: The researchers developed an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2, capable of detecting as low as 1 copy/mu L of N and S genes in clinical samples. The sensor showed a 100% concordance with qRT-PCR results in clinical sample evaluation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Qianxin Wu et al.
Summary: INSIGHT is a two-stage COVID-19 testing strategy that combines point-of-care diagnosis with next-generation sequencing to achieve population-scale testing; the first stage provides quick decentralized readout, aiming for early isolation of asymptomatic patients and gives results within 1 to 2 hours; the second stage confirms near-patient testing results and facilitates centralized data collection.
Article
Chemistry, Multidisciplinary
Jing Wang et al.
Summary: A highly sensitive ratiometric fluorescent lateral flow immunoassay strip was developed for the rapid and accurate detection of the acute myocardial infarction biomarker, H-FABP. By utilizing a custom smartphone-based analytical device and tonality analysis, quantitative detection of H-FABP with a low limit of detection was achieved. The visually distinguishable color change on the strip around the threshold concentration of H-FABP enables semi-quantitative diagnosis by the naked eye.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Microbiology
Jian Zhang et al.
Summary: CRISPR-based diagnostic technologies show great potential in pathogen detection due to their high sensitivity and specificity. Efforts are being made to improve the CRISPR trans-cleavage efficiencies and signal detection sensitivities, while challenges in detection sensitivity and cost of amplification-free systems are also being addressed.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Catherine A. Freije et al.
Summary: Despite the lack of diagnostic and therapeutic technologies, CRISPR-Cas systems show potential in addressing critical needs against infectious diseases, especially in viral diagnostics and therapies. Technologies related to CRISPR-Cas, particularly DNA-targeting Cas12 and RNA-targeting Cas13, are being harnessed for these purposes with accelerated development in response to the COVID-19 pandemic.
CELL HOST & MICROBE
(2021)
Article
Multidisciplinary Sciences
Nicholas G. Davies et al.
Summary: Studies have shown that the SARS-CoV-2 B.1.1.7 lineage is more transmissible and may cause more severe illness compared to pre-existing variants.
Letter
Medicine, General & Internal
Salim S. Abdool Karim et al.
Summary: The emergence of SARS-CoV-2 variants, including B.1.1.7 (VOC-202012/01), 501Y.V2 (B.1.351), and P.1 (B.1.1.28.1), has raised concerns about worsening Covid-19 and potential escape from vaccine-induced immunity.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Editorial Material
Medicine, General & Internal
Adam S. Lauring et al.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2021)
Review
Microbiology
William T. Harvey et al.
Summary: The evolution of SARS-CoV-2 has been characterized by the emergence of mutations and variants that impact virus characteristics. Manufacturers are preparing for possible updates to vaccines in response to changes in the virus population, and it is crucial to monitor genetic and antigenic changes alongside experiments to understand the impacts of mutations.
NATURE REVIEWS MICROBIOLOGY
(2021)
Editorial Material
Multidisciplinary Sciences
Omar O. Abudayyeh et al.
Article
Biochemistry & Molecular Biology
Fan Hong et al.
Article
Chemistry, Analytical
Huixin Zhang et al.
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Annika Krueger et al.
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Fengqin Li et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Multidisciplinary
Linda J. Carter et al.
ACS CENTRAL SCIENCE
(2020)
Article
Biotechnology & Applied Microbiology
James P. Broughton et al.
NATURE BIOTECHNOLOGY
(2020)
Article
Microbiology
Chantal B. F. Vogels et al.
NATURE MICROBIOLOGY
(2020)
Letter
Medicine, General & Internal
Julia Joung et al.
NEW ENGLAND JOURNAL OF MEDICINE
(2020)
Article
Multidisciplinary Sciences
Xiong Ding et al.
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Yifan Dai et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Multidisciplinary
Jidong Shi et al.
ADVANCED MATERIALS
(2019)
Article
Multidisciplinary Sciences
Jonathan S. Gootenberg et al.
