期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 62, 期 23, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202301696
关键词
Gryme B Protease; NIR-II Fluorescence; Ratio Imaging; Tralant Rejection
Early diagnosis of allograft rejection is essential for improving immune-related treatment of transplant recipients. This study introduces a non-invasive, fast and sensitive near-infrared fluorescent nanosensor (ErGZ) for early detection of rejection. The sensor can accurately detect granzyme B, a biomarker overexpressed during rejection, allowing for early diagnosis of rejection in skin and deep buried islets transplant mice models.
Early diagnosis of allograft rejection helps to improve the immune-related management of transplant recipients. The clinically-used core needle biopsy method is invasive and subject to sampling error. In vivo fluorescence imaging for monitoring immune-related processes has the advantages of non-invasiveness, fast feedback and high sensitivity. Herein, we report a responsive second near-infrared (NIR-II) fluorescent nanosensor (ErGZ) to detect early allograft rejection. ErGZ allows ratiometric in vivo fluorescence sensing of granzyme B, which is overexpressed in recipients' T cells during the onset of rejection. The sensor demonstrates efficacious detection of allograft rejection with high sensitivity and specificity, which accomplishes non-invasive diagnosis of rejection in skin and deep buried islets transplant mice models 2 d and 5 d earlier than biopsy, by in vivo fluorescence imaging and urinary detection, respectively, providing a valuable approach for therapeutical management.
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