Ferroptosis Detection: From Approaches to Applications

Understanding the intricate molecular machinery that governs ferroptosis and leveraging this accumulating knowledge could facilitate disease prevention, diagnosis, treatment, and prognosis. Emerging approaches for the in situ detection of the major regulators and biological events across cellular, tissue, and in living subjects provide a multiscale perspective for studying ferroptosis. Furthermore, advanced applications that integrate ferroptosis detection and the latest technologies hold tremendous promise in ferroptosis research. In this review, we first briefly summarize the mechanisms and key regulators underlying ferroptosis. Ferroptosis detection approaches are then presented to delineate their design, mechanisms of action, and applications. Special interest is placed on advanced ferroptosis applications that integrate multifunctional platforms. Finally, we discuss the prospects and challenges of ferroptosis detection approaches and applications, with the aim of providing a roadmap for the theranostic development of a broad range of ferroptosis-related diseases.

Automated summary

Understanding the intricate molecular machinery of ferroptosis and leveraging this knowledge can advance disease prevention, diagnosis, treatment, and prognosis. New approaches for in situ detection of key regulators and events provide a comprehensive view of ferroptosis across different scales. Advanced applications integrating ferroptosis detection with cutting-edge technologies show great potential in ferroptosis research. This review summarizes the mechanisms and regulators of ferroptosis, presents detection approaches and applications, and discusses future prospects and challenges for therapeutic development.