期刊
ANALYTICAL CHEMISTRY
卷 95, 期 10, 页码 4786-4794出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.3c00101
关键词
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In this study, we developed a PUF protein-based luciferase reporter system called PUF/miR, which can quantitatively and non-invasively detect miRNA activity in living cells and animal models. The feasibility of this system was validated by monitoring the expression of several types of miRNAs in different cell types and tissues in mice. The reliability and quantitative consistency of the system were further confirmed by quantitative RT-PCR. This innovative approach provides a potential means for noninvasive monitoring of disease-related miRNAs and can contribute to a deeper understanding of miRNA biology.
Precise characterization of miRNA expression patterns is critical to exploit the complexity of miRNA regulation in biology. Herein, we developed a Pumilio/FBF (PUF) protein -based engineering luciferase reporter system, PUF/miR, to quantitatively and non-invasively sense miRNA activity in living cells and animal models. We verified the feasibility of this reporter by monitoring the expression of several types of miRNAs (miRNA-9, 124a, 1, and 133a) in neural and muscle differentiated cells as well as subcutaneous or tibial anterior muscles in mice. The quantitative RT-PCR also validated the reliability and quantitative consistency of bioluminescence imaging in detecting miRNA expression. We further effectively employed this reporter system to visualize the expression of miRNA-1 and miRNA-133a in mouse models of skeletal muscle injury. As a non-invasive and convenient innovative approach, our results have realized the positive bioluminescence imaging of endogenous miRNAs in vitro and in vivo using the PUF/miR system. We believe that this approach would provide a potential means for noninvasive monitoring of disease-related miRNAs and could facilitate a deeper understanding of miRNA biology.
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