Aggregation-Induced Electrochemiluminescence Based on a Zinc- Based Metal-Organic Framework and a Double Quencher Au@UiO-66-NH2 for the Sensitive Detection of Amyloid ? 42 via Resonance Energy Transfer

A novel sandwich electrochemiluminescence (ECL) biosensor based on aggregation-induced electrochemiluminescence resonance energy transfer (AIECL-RET) was designed for the sensitive detection of amyloid fl42 (Afl42). The synthesized silver nanoparticle-functionalized zinc metal-organic framework (Ag@ ZnPTC) and gold nanoparticle-functionalized zirconium organic framework (Au@UiO-66-NH2) were used as the ECL donor and acceptor, respectively. AgNPs were generated in situ on the surface of ZnPTC, which further improved the ECL intensity and the loading of antibody 1 (Ab1). Under the optimized experimental conditions, the linear detection range of Afl42 concentration was 10 fg/mL to 100 ng/mL, and the detection limit was 2.4 fg/mL (S/N = 3). The recoveries of Afl42 were 99.5-104%. The method has good stability, repeatability, and specificity. Ag@ZnPTC/Au@UiO-66-NH2 provides an assay for the sensitive detection of disease biomarkers.

Automated summary

A novel sandwich electrochemiluminescence biosensor was developed for the sensitive detection of Afl42 using aggregation-induced electrochemiluminescence resonance energy transfer. The biosensor utilized silver nanoparticle-functionalized zinc metal-organic framework as the ECL donor and gold nanoparticle-functionalized zirconium organic framework as the acceptor. The method exhibited a linear detection range of 10 fg/mL to 100 ng/mL and a detection limit of 2.4 fg/mL. It also demonstrated good stability, repeatability, and specificity, making it a promising tool for disease biomarker detection.