4.7 Article

Quantification methods of determining brewer's and pharmaceutical yeast cell viability: accuracy and impact of nanoparticles

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemical Research Methods

Studying the impact of cell age on the yeast growth behaviour of Saccharomyces pastorianus var. carlsbergensis by magnetic separation

Marco Eigenfeld et al.

Summary: Despite the wide use of yeast in various industries, the impact of viability and age distribution on cultivation performance is not fully understood. In this study, a magnetic batch separation method was used to isolate daughter and mother cells from a heterogeneous culture, revealing that low viability cultures with high daughter cell content perform similarly to high viability cultures with low daughter cell content. The separated daughter cells showed higher growth rates than mother cells in both aerobic and anaerobic conditions. These findings highlight the importance of viability and age in yeast cultivation and lay the foundation for improving yeast-based processes.

BIOTECHNOLOGY JOURNAL (2023)

Article Biochemistry & Molecular Biology

Impacts of Magnetic Immobilization on the Growth and Metabolic Status of Recombinant Pichia pastoris

Seyedeh-Masoumeh Tagizadeh et al.

Summary: Magnetic immobilization is a viable technique that can be effectively applied in eukaryotic expression systems without adverse effects on cell growth and metabolic status.

MOLECULAR BIOTECHNOLOGY (2022)

Article Chemistry, Multidisciplinary

Application of magnetic immboilization for ethanol biosynthesis using Saccharomyces cerevisiae

Farid Reza Firoozi et al.

Summary: The study demonstrates that immobilizing S. cerevisiae cells with L-lysine-coated magnetite nanoparticles is a promising method for ethanol production, with magnetically immobilized yeast cells successfully separated using an integrated magnetic separation process.

SEPARATION SCIENCE AND TECHNOLOGY (2022)

Article Biochemical Research Methods

Autofluorescence prediction model for fluorescence unmixing and age determination

Marco Eigenfeld et al.

Summary: This study compared principal component regression (PCR) and random forest (RF) for predicting autofluorescence signals in flow cytometry. RF outperformed PCR and was found to be better suited for signal unmixing. The subsequent Gaussian mixture analysis based on RF showed better agreement with microscopy data for age determination of cells, compared to the PCR-based method. This approach allows for fast and reliable unmixing of flow cytometric spectral data and enables age determination of cells in industrial processes.

BIOTECHNOLOGY JOURNAL (2022)

Review Medical Laboratory Technology

Sample size, power and effect size revisited: simplified and practical approaches in pre-clinical, clinical and laboratory studies

Ceyhan Ceran Serdar et al.

Summary: Calculating sample size in scientific studies is crucial for scientific contribution, impacting hypothesis and study design. Incorrect sample size may lead to inadequate results, time and cost losses, and ethical issues. This review aims to highlight the importance of sample size and provide researchers with tools to estimate it accurately.

BIOCHEMIA MEDICA (2021)

Article Cell Biology

Plasma Membrane Fluidity: An Environment Thermal Detector in Plants

Dora L. Cano-Ramirez et al.

Summary: The lipid matrix in cell membranes is dynamic and plays a role in membrane fluidity changes in response to temperature fluctuation. The plasma membrane (PM) of plants is highly fluid and sensitive to temperature variations, serving as an ideal temperature detector with the ability to quickly adjust in response to small temperature changes. This makes the PM an optimal alternative for temperature detection in plants.
Article Chemistry, Medicinal

Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin

Chiara Turrina et al.

Summary: This study investigates the binding patterns of the peptide lasioglossin III from bee venom on bare IONs, revealing that bare IONs are an interesting platform material for the development of drug-delivery carriers for cationic peptides.

PHARMACEUTICALS (2021)

Article Engineering, Chemical

Generation of Yeast Protoplasts by Lytic Actions of Iron Oxide Magnetic Nanoparticles

Menglin Xiao et al.

Summary: The preparation of protoplasts of Saccharomyces cerevisiae and Pichia pastoris using Fe3O4 MNPs was successful, with characterization revealing cell wall breakage and allowing for regeneration, DNA extraction, and transformation. This approach provides a feasible platform for genetic manipulation of yeasts and offers potential for yeast-based biotechnological applications, with the possibility of extension to other microbiomes of industrial importance.

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2021)

Article Multidisciplinary Sciences

Analysis of methods for quantifying yeast cell concentration in complex lignocellulosic fermentation processes

Ruifei Wang et al.

Summary: Cell mass and viability play a crucial role in the productivity of fermentation processes, especially in lignocellulosic media. Various methods were evaluated for quantifying yeast cell concentrations, with manual cell enumeration and colony forming unit enumeration proving to be the most reliable in lignocellulosic conditions.Developing reliable cell quantification methods in lignocellulosic contexts will further optimize and control fermentation processes.

SCIENTIFIC REPORTS (2021)

Article Chemistry, Multidisciplinary

Adsorption of Organic Dyes on Magnetic Iron Oxide Nanoparticles. Part I: Mechanisms and Adsorption-Induced Nanoparticle Agglomeration

Delphine Talbot et al.

Summary: This study investigates the effect of organic dye adsorption on the surface of nanoparticles on primary and secondary agglomeration, with the main findings being that dye adsorption is largely driven by electrostatic interactions and is sensitive to pH and ionic strength variations. The primary agglomeration of nanoparticles may result from interactions between adsorbed dye molecules. In terms of application, citrated nanoparticles show promise for water treatment with good reusability, maintaining an adsorption efficiency of around 99% after nine cycles.

ACS OMEGA (2021)

Article Chemistry, Multidisciplinary

Recombinant protein linker production as a basis for non-invasive determination of single-cell yeast age in heterogeneous yeast populations

Marco Eigenfeld et al.

Summary: Research has shown that a specific protein binding technique allows for rapid measurement of yeast population dynamics using flow cytometry, enabling qualitative determination of yeast cell age distribution.

RSC ADVANCES (2021)

Review Biochemistry & Molecular Biology

Iron-based nanoparticles and their potential toxicity: Focus on oxidative stress and apoptosis

Jovana Paunovic et al.

CHEMICO-BIOLOGICAL INTERACTIONS (2020)

Article Biochemistry & Molecular Biology

ROS-independent toxicity of Fe3O4 nanoparticles to yeast cells: Involvement of mitochondrial dysfunction

Qi Peng et al.

CHEMICO-BIOLOGICAL INTERACTIONS (2018)

Article Multidisciplinary Sciences

Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae

Imelda Galvin Marquez et al.

PLOS ONE (2018)

Article Multidisciplinary Sciences

The generational scalability of single-cell replicative aging

Ping Liu et al.

SCIENCE ADVANCES (2018)

Article Biotechnology & Applied Microbiology

Membrane fluidification by ethanol stress activates unfolded protein response in yeasts

Elisabet Navarro-Tapia et al.

MICROBIAL BIOTECHNOLOGY (2018)

Article Biochemistry & Molecular Biology

Toxicity of iron oxide nanoparticles: Size and coating effects

Maxim A. Abakumov et al.

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2018)

Article Biotechnology & Applied Microbiology

Further investigation of relationships between membrane fluidity and ethanol tolerance in Saccharomyces cerevisiae

Safri Ishmayana et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2017)

Article Biochemical Research Methods

Yeast cell fractionation by morphology in dilute ferrofluids

Qi Chen et al.

BIOMICROFLUIDICS (2017)

Article Cell Biology

Aging yeast gain a competitive advantage on non-optimal carbon sources

Stephen Frenk et al.

AGING CELL (2017)

Article Biotechnology & Applied Microbiology

Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted

Shaopan Bao et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2015)

Editorial Material Biochemistry & Molecular Biology

Turning Up the Heat on Membrane Fluidity

Weiwei Fan et al.

Article Biochemistry & Molecular Biology

Magnetically responsive yeast cells: methods of preparation and applications

Ivo Safarik et al.

Article Biotechnology & Applied Microbiology

A new method for the rapid separation of magnetized yeast in sparkling wine

Mann Berovic et al.

BIOCHEMICAL ENGINEERING JOURNAL (2014)

Article Biochemistry & Molecular Biology

Isolation and characterization of the plasma membrane from the yeast Pichia pastoris

Karlheinz Grillitsch et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2014)

Article Biotechnology & Applied Microbiology

Comparison of methods used for assessing the viability and vitality of yeast cells

Magdalena Kwolek-Mirek et al.

FEMS YEAST RESEARCH (2014)

Article Materials Science, Multidisciplinary

Self-organization of magnetite nanoparticles in providing Saccharomyces cerevisiae Yeasts with magnetic properties

S. V. Gorobets et al.

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS (2013)

Article Biotechnology & Applied Microbiology

One-step magnetic modification of yeast cells by microwave-synthesized iron oxide microparticles

K. Pospiskova et al.

LETTERS IN APPLIED MICROBIOLOGY (2013)

Article Biotechnology & Applied Microbiology

Rapid flow cytometric method for viability determination of solventogenic clostridia

Michaela Linhova et al.

FOLIA MICROBIOLOGICA (2012)

Article Biochemistry & Molecular Biology

Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction

Jin-Ying Lu et al.

Article Chemistry, Multidisciplinary

Oxidative Stress and Apoptosis Induced by Iron Oxide Nanoparticles in Cultured Human Umbilical Endothelial Cells

Mo-Tao Zhu et al.

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY (2010)

Review Microbiology

Yeast responses to stresses associated with industrial brewery handling

Brian R. Gibson et al.

FEMS MICROBIOLOGY REVIEWS (2007)

Article Biotechnology & Applied Microbiology

Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena

R Weis et al.

FEMS YEAST RESEARCH (2004)

Article Biochemistry & Molecular Biology

Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order

FM Harris et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2002)

Review Biochemistry & Molecular Biology

Regulation of plasma membrane H+-ATPase in fungi and plants

F Portillo

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES (2000)