期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 -, 期 -, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-023-04663-1
关键词
AChE; CeO2 nanoparticles; Photothermal effect; Organophosphorus; POCT; Liver injury
In this study, a colorimetric and photothermal dual-signal point-of-care testing (POCT) sensing platform based on CeO2-TMB was developed for visualizing acetylcholinesterase (AChE) activity in liver-injured mice. This method compensates for false positives of single signal detection, enabling rapid, low-cost portable detection of AChE. Furthermore, the CeO2-TMB sensing platform allows for the diagnosis of liver injury and provides an effective tool for studying liver disease in basic medicine and clinical applications.
Acetylcholinesterase (AChE), a crucial enzyme related to liver function, is involved in numerous physiological processes such as neurotransmission and muscular contraction. The currently reported techniques for detecting AChE mainly rely on a single signal output, limiting their high-accuracy quantification. The few reported dual-signal assays are challenging to implement in dual-signal point-of-care testing (POCT) because of the need for large instruments, costly modifications, and trained operators. Herein, we report a colorimetric and photothermal dual-signal POCT sensing platform based on CeO2-TMB (3,3',5,5'-tetramethylbenzidine) for the visualization of AChE activity in liver-injured mice. The method compensates for the false positives of a single signal and realizes the rapid, low-cost portable detection of AChE. More importantly, the CeO2-TMB sensing platform enables the diagnosis of liver injury and provides an effective tool for studying liver disease in basic medicine and clinical applications.
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