期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 138, 期 49, 页码 15809-15812出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b08671
关键词
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资金
- Army Research Office [W911NF-09-0001]
- W. M. Keck Foundation
- Swiss National Science Foundation
- Early Postdoc Mobility fellowship
The continuous, real-time monitoring of specific molecular targets in unprocessed clinical samples would enable many transformative medical applications. Electrochemical aptamer-based (E-AB) sensors appear to be a promising approach to this end because of their selectivity (performance in complex samples, such as serum) and reversible, single-step operation. E-AB sensors suffer, however, from often-severe baseline drift when challenged in undiluted whole blood. In response we report here a dual-reporter approach to performing E-AB baseline drift correction. The approach incorporates two redox reporters on the aptamer, one of which serves as the target-responsive sensor and the other, which reports at a distinct, nonoverlapping redox potential, serving as a drift correcting reference. Taking the difference in their relative signals largely eliminates the drift observed for these sensors in flowing, undiluted whole blood, reducing drift of up to 50% to less than 2% over many hours of continuous operation under these challenging conditions.
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