期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 138, 期 33, 页码 10382-10385出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b04799
关键词
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资金
- National Natural Science Foundation of China [81330077, 91213302, 21272291]
- Guangdong Natural Science Funds for Distinguished Young Scholar [2015A030306004]
- Guangdong Provincial Key Laboratory of Construction Foundation [2011A060901014]
The RNA G-quadruplex is an important secondary structure formed by guanine-rich RNA sequences. However, its folding studies have mainly been studied in vitro. Accurate identification of RNA G-quadruplex formation within a sequence of interest remains difficult in cells. Herein, and based on the guanine-rich sequence in the 5'-UTR of NRAS mRNA, we designed and synthesized the first G-quadruplex-triggered fluorogenic hybridization (GTFH) probe, ISCH-nras1, for the unique visualization of the G-quadruplexes that form in this region. ISCH-nras1 is made up of two parts: The first is a fluorescent light-up moiety specific to G-quadruplex structures, and the second is a DNA molecule that can hybridize with a sequence that is adjacent to the guanine rich sequence in the NRAS mRNA 5'-UTR. Further evaluation studies indicated that ISCH-nras1 could directly and precisely detect the targeted NRAS RNA G-quadruplex structures, both in vitro and in cells. Thus, this GTFH probe was a useful tool for directly investigating the folding of G-quadruplex structures within an RNA of interest and represents a new direction for the design of smart RNA G-quadruplex probes.
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