期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 138, 期 31, 页码 10047-10054出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b06508
关键词
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资金
- NIH, National Institute of General Medical Sciences
- HHMI
- U.S. Department of Energy (DOE) [DE-AC02-05CH11231]
- Office of Biological and Environmental Research of the DOE
- Office of Basic Energy Sciences of the DOE
- National Center for Research Resources [P41RR012408]
- National Institute of General Medical Sciences of the NIH [P41GM103473]
- DOE Office of Science [DE-AC02-06CH11357]
- NSF [1360635, 1334109, EFRI-1332411, CCP-1526650]
- ARO [W911NF-12-1-0420]
- NIH [R01GM104960]
- Presidential Strategic Initiative Fund from Arizona State University
- MURI from the ARO [W911NF-11-1-0024]
- ONR [N000141110729]
- DOE [DE-SC0007991]
- Gordon and Betty Moore Foundation [GBMF3849]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1360635] Funding Source: National Science Foundation
Structural DNA nanotechnology combines branched DNA junctions with sticky-ended cohesion to create self-assembling macromolecular architectures. One of the key goals of structural DNA nanotechnology is to construct three-dimensional (3D) crystalline lattices. Here we present a new DNA motif and a strategy that has led to the assembly of a 3D lattice. We have determined the X-ray crystal structures of two related constructs to 3.1 A resolution using bromine-derivatized crystals. The motif we used employs a five nucleotide repeating sequence that weaves through a series of two-turn DNA duplexes. The duplexes are tied into a layered structure that is organized and dictated by a concert of four-arm junctions; these in turn assemble into continuous arrays facilitated by sequence-specific sticky-ended cohesion. The 3D X-ray structure of these DNA crystals holds promise for the design of new structural motifs to create programmable 3D DNA lattices with atomic spatial resolution. The two arrays differ by the use of four or six repeats of the five-nucleotide units in the repeating but statistically disordered central strand. In addition, we report a 2D rhombuslike array formed from similar components.
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