Engineering bacteria to modulate host metabolism

Article Substance Abuse

Engineered bacteria producing aryl-hydrocarbon receptor agonists protect against ethanol-induced liver disease in mice

Tetsuya Kouno et al.

Summary: This study demonstrates that engineering gut bacteria to produce tryptophan metabolites can alleviate liver disease caused by alcohol through Ahr activation in intestinal immune cells.

ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH (2023)

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Article Biotechnology & Applied Microbiology

Engineering of a probiotic yeast for the production and secretion of medium-chain fatty acids antagonistic to an opportunistic pathogen Candida albicans

Hua Ling et al.

Summary: In this study, a commercial boulardii strain was engineered to produce medium-chain fatty acids (MCFAs) with antagonistic effects against Candida albicans. The engineered boulardii strain constitutively produced and secreted a mixture of C6:0, C8:0, and C10:0, which reduced biofilm and hyphal formations in C. albicans. The secreted MCFAs also upregulated the expression of virulence-related genes and immune response genes.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2023)

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Editorial Material Microbiology

Editorial: The Role of Mobile Genetic Elements in Bacterial Evolution and Their Adaptability

Filipa F. Vale et al.

FRONTIERS IN MICROBIOLOGY (2022)

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Review Gastroenterology & Hepatology

Promises of microbiome-based therapies

Jasmohan S. Bajaj et al.

Summary: This review discusses the importance of manipulating the gut microbiota as a novel treatment approach for liver diseases. It highlights the changes in intestinal microbiota and gut barrier dysfunction in patients with liver disease. Preclinical models demonstrate the significant role of gut microbiota in the pathogenesis of various liver diseases.

JOURNAL OF HEPATOLOGY (2022)

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Article Biochemistry & Molecular Biology

An engineered bacterial therapeutic lowers urinary oxalate in preclinical models and in silico simulations of enteric hyperoxaluria

David Lubkowicz et al.

Summary: Enteric hyperoxaluria (EH) is a metabolic disease without approved pharmaceutical treatments. SYNB8802, an engineered bacterial therapeutic, shows promise in reducing urinary oxalate excretion in animal models. Mathematical modeling predicts a clinically meaningful lowering of urinary oxalate excretion in humans, suggesting that SYNB8802 could be a potential treatment for EH.

MOLECULAR SYSTEMS BIOLOGY (2022)

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Article Microbiology

Positive Interventional Effect of Engineered Butyrate-Producing Bacteria on Metabolic Disorders and Intestinal Flora Disruption in Obese Mice

Lina Wang et al.

Summary: This study demonstrated that continuous consumption of engineered butyrate-producing bacteria had a significant inhibitory effect on obesity induced by a high-fat diet. The bacteria not only improved glucose and lipid metabolism but also regulated gut microbe composition. This research provided a new strategy for the effective and convenient treatment of obesity in the long term.

MICROBIOLOGY SPECTRUM (2022)

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Article Biochemistry & Molecular Biology

Intestinal transgene delivery with native E. coli chassis allows persistent physiological changes

Baylee J. Russell et al.

Summary: This study demonstrates the potential of using native bacteria as chassis for transgene delivery to manipulate the gut microbiome and reverse pathology in conventionally raised hosts.

CELL (2022)

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Review Biochemistry & Molecular Biology

Synthetic chromosomes, genomes, viruses, and cells

J. Craig Venter et al.

Summary: Synthetic genomics involves synthesizing complete genomes or chromosomes to create viruses, bacteria, and eukaryotic cells. This field has revolutionized viral reverse genetics, vaccine design, and bacterial resistance. Future advances in DNA synthesis will open up extraordinary opportunities in medicine, industry, agriculture, and research.

CELL (2022)

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Editorial Material Endocrinology & Metabolism

What's preventing us from curbing the obesity crisis?

[Anonymous]

LANCET DIABETES & ENDOCRINOLOGY (2022)

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Review Microbiology

Genetically engineered bacterium: Principles, practices, and prospects

Yiting Liu et al.

Summary: Advances in synthetic biology and bacteriotherapy have enabled the use of genetically engineered bacteria (GEB) for disease treatment. GEB can continuously produce proteins and compounds in the body, diagnosing or treating diseases and acting as adjuvants through immune system regulation. However, practical implementation of this therapeutic modality remains a major challenge.

FRONTIERS IN MICROBIOLOGY (2022)

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Review Biotechnology & Applied Microbiology

Microbiome engineering: engineered live biotherapeutic products for treating human disease

Jack W. Rutter et al.

Summary: The human microbiota is associated with many diseases, and microbiome engineering methods aim to modify the composition and function of the microbiota for therapeutic purposes. Clinical studies on engineered microorganisms have shown promising progress in recent years.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

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Article Medicine, General & Internal

Tirzepatide Once Weekly for the Treatment of Obesity

Ania M. Jastreboff et al.

Summary: In this 72-week trial in participants with obesity, once-weekly doses of 5 mg, 10 mg, or 15 mg of tirzepatide provided substantial and sustained reductions in body weight, and improvements in cardiometabolic measures were also observed.

NEW ENGLAND JOURNAL OF MEDICINE (2022)

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Review Biotechnology & Applied Microbiology

Engineered Bacteria-Based Living Materials for Biotherapeutic Applications

Rabia Omer et al.

Summary: Future advances in therapeutics require the development of dynamic and intelligent living materials. Engineered living materials (ELMs) play a vital role in biotherapeutic applications and serve as a new platform for treating intractable diseases. Synthetic biology is crucial in designing and creating genetically engineered living materials, particularly bacteria. This review highlights the recent advances in engineered bacterial-based therapies, focusing on cancer, inflammatory bowel diseases, and infection treatments.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

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Review Microbiology

Gut microbiota in human metabolic health and disease

Yong Fan et al.

Summary: The intestinal microbiota plays a significant role in human metabolic health, potentially contributing to the development of common metabolic disorders when dysregulated. Current research is shifting towards cause-and-effect studies and utilizing high-throughput human multi-omics data to identify potential molecular mechanisms behind these associations.

NATURE REVIEWS MICROBIOLOGY (2021)

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Article Immunology

Engineered Bacteria of MG1363-pMG36e-GLP-1 Attenuated Obesity-Induced by High Fat Diet in Mice

Lingfang Wang et al.

Summary: The genetically engineered bacteria secreting GLP-1 can effectively improve obesity by promoting fatty acid oxidation and increasing intestinal microbial diversity in obese mice induced by high fat diet.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

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Article Multidisciplinary Sciences

Improvement of a synthetic live bacterial therapeutic for phenylketonuria with biosensor-enabled enzyme engineering

Kristin J. Adolfsen et al.

Summary: In this study, a more potent EcN-based PKU strain was developed through the optimization of whole cell PAL activity, showcasing approximately two-fold increase in in vivo PAL activity compared to the previous strain. The biosensor-based ultra-high-throughput screening approach utilized in this study could potentially lead to improved in vivo performance for PKU treatment in the future.

NATURE COMMUNICATIONS (2021)

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Article Medicine, General & Internal

A Placebo-Controlled Trial of Subcutaneous Semaglutide in Nonalcoholic Steatohepatitis

Philip N. Newsome et al.

Summary: This phase 2 trial demonstrated that treatment with semaglutide resulted in a significantly higher percentage of patients with NASH resolution compared to placebo in patients with NASH, but did not show a significant between-group difference in the percentage of patients with an improvement in fibrosis stage.

NEW ENGLAND JOURNAL OF MEDICINE (2021)

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Article Biotechnology & Applied Microbiology

Antidiabetic effect of an engineered bacterium Lactobacillus plantarum-pMG36e-GLP-1 in monkey model

Jie Luo et al.

Summary: Engineered strain Lactobacillus plantarum-pMG36e-GLP-1 was found to significantly reduce postprandial hyperglycaemia in T2DM monkeys. The study showed that this strain impacted the gut microbial composition and faecal metabolomic profile, indicating its potential as a novel candidate for diabetes treatment.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2021)

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Review Endocrinology & Metabolism

The gut-brain axis: regulating energy balance independent of food intake

Ruben Nogueiras

Summary: Obesity is a global epidemic that poses significant health and economic challenges worldwide. Recent research has highlighted the role of gut hormones in regulating energy balance, feeding behavior, energy expenditure, and weight loss. Some of these hormones can act on the hypothalamus independently of food intake to modulate thermogenesis and adiposity.

EUROPEAN JOURNAL OF ENDOCRINOLOGY (2021)

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Article Medicine, General & Internal

Tirzepatide versus Semaglutide Once Weekly in Patients with Type 2 Diabetes

Juan P. Frias et al.

Summary: The study showed that Tirzepatide was both noninferior and superior to Semaglutide in terms of the mean change in glycated hemoglobin levels for patients with type 2 diabetes over 40 weeks. Additionally, reductions in body weight were greater with Tirzepatide compared to Semaglutide, with similar rates of gastrointestinal adverse events reported in both groups.

NEW ENGLAND JOURNAL OF MEDICINE (2021)

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Review Microbiology

Bile Salt Hydrolases: At the Crossroads of Microbiota and Human Health

Melanie Bourgin et al.

Summary: The gut microbiota has been increasingly linked to metabolic health and disease, with bile salt hydrolase (BSH) playing a key role in both microbial and host physiology. Understanding the role of BSH in health can pave the way for new therapeutic targets in human diseases.

MICROORGANISMS (2021)

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Article Endocrinology & Metabolism

Safety and pharmacodynamics of an engineered E. coli Nissle for the treatment of phenylketonuria: a first-in-human phase 1/2a study

Marja K. Puurunen et al.

Summary: The engineered E. coli strain SYNB1618 shows potential for the treatment of PKU, as it was safe and well tolerated in a phase 1/2a clinical trial with both healthy volunteers and PKU patients. The study demonstrated dose-responsive increases in strain-specific Phe metabolites in plasma and urine, supporting the proof of concept for using engineered bacteria in the treatment of rare metabolic disorders.

NATURE METABOLISM (2021)

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Article Food Science & Technology

Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification

Jing Lu et al.

FOOD AND CHEMICAL TOXICOLOGY (2020)

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Article Endocrinology & Metabolism

Oral butyrate does not affect innate immunity and islet autoimmunity in individuals with longstanding type 1 diabetes: a randomised controlled trial

Pieter F. de Groot et al.

DIABETOLOGIA (2020)

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Article Biochemistry & Molecular Biology

Resilient living materials built by printing bacterial spores

Lina M. Gonzalez et al.

NATURE CHEMICAL BIOLOGY (2020)

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Review Biochemical Research Methods

Engineering commensal bacteria to rewire host-microbiome interactions

In Young Hwang et al.

CURRENT OPINION IN BIOTECHNOLOGY (2020)

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Article Multidisciplinary Sciences

Developing a new class of engineered live bacterial therapeutics to treat human diseases

Mark R. Charbonneau et al.

NATURE COMMUNICATIONS (2020)

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Article Biotechnology & Applied Microbiology

Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice

Bai Liang et al.

MICROBIAL CELL FACTORIES (2020)

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Review Microbiology

Microbiota and Fatty Liver Disease - the Known, the Unknown, and the Future

Sonja Lang et al.

CELL HOST & MICROBE (2020)

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Article Microbiology

Secretion of Recombinant Interleukin-22 by Engineered Lactobacillus reuteri Reduces Fatty Liver Disease in a Mouse Model of Diet-Induced Obesity

Jee-Hwan Oh et al.

MSPHERE (2020)

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Review Biochemistry & Molecular Biology

Live biotherapeutic products: the importance of a defined regulatory framework

Magali Cordaillat-Simmons et al.

EXPERIMENTAL AND MOLECULAR MEDICINE (2020)

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Review Microbiology

Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics

Ursula Fels et al.

FRONTIERS IN MICROBIOLOGY (2020)

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Article Gastroenterology & Hepatology

Bacteria engineered to produce IL-22 in intestine induce expression of REG3G to reduce ethanol-induced liver disease in mice

Tim Hendrikx et al.

GUT (2019)

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Article Cell Biology

An engineered E-coli Nissle improves hyperammonemia and survival in mice and shows dose-dependent exposure in healthy humans

Caroline B. Kurtz et al.

SCIENCE TRANSLATIONAL MEDICINE (2019)

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Article Toxicology

Lyophilized B. subtilis ZB183 Spores: 90-Day Repeat Dose Oral (Gavage) Toxicity Study in Wistar Rats

B. Appala Naidu et al.

JOURNAL OF TOXICOLOGY (2019)

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Review Microbiology

Engineering bacteria for diagnostic and therapeutic applications

David T. Riglar et al.

NATURE REVIEWS MICROBIOLOGY (2018)

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Article Biochemistry & Molecular Biology

Personalized Gut Mucosal Colonization Resistance to Empiric Probiotics Is Associated with Unique Host and Microbiome Features

Niv Zmora et al.

CELL (2018)

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Article Biotechnology & Applied Microbiology

Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria

Vincent M. Isabella et al.

NATURE BIOTECHNOLOGY (2018)

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Article Medicine, General & Internal

Alcoholic liver disease

Helmut K. Seitz et al.

NATURE REVIEWS DISEASE PRIMERS (2018)

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Article Biochemistry & Molecular Biology

Tunable thermal bioswitches for in vivo control of microbial therapeutics

Dan I. Piraner et al.

NATURE CHEMICAL BIOLOGY (2017)

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Article Multidisciplinary Sciences

Complex cellular logic computation using ribocomputing devices

Alexander A. Green et al.

NATURE (2017)

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Review Pharmacology & Pharmacy

Microbiome therapeutics - Advances and challenges

Mark Mimee et al.

ADVANCED DRUG DELIVERY REVIEWS (2016)

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Article Multidisciplinary Sciences

Synthetic recombinase-based state machines in living cells

Nathaniel Roquet et al.

SCIENCE (2016)

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Article Endocrinology & Metabolism

Engineered Commensal Bacteria Reprogram Intestinal Cells Into Glucose-Responsive Insulin-Secreting Cells for the Treatment of Diabetes

Franklin F. Duan et al.

DIABETES (2015)

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Review Endocrinology & Metabolism

Short-chain fatty acids in control of body weight and insulin sensitivity

Emanuel E. Canfora et al.

NATURE REVIEWS ENDOCRINOLOGY (2015)

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Review Gastroenterology & Hepatology

Pathogenesis of Hepatic Encephalopathy: Role of Ammonia and Systemic Inflammation

Dominic R. Aldridge et al.

JOURNAL OF CLINICAL AND EXPERIMENTAL HEPATOLOGY (2015)

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Article Multidisciplinary Sciences

Interleukin-22 alleviates metabolic disorders and restores mucosal immunity in diabetes

Xiaoting Wang et al.

NATURE (2014)

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Article Gastroenterology & Hepatology

The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic

Colin Hill et al.

NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY (2014)

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Article Multidisciplinary Sciences

Genomically Recoded Organisms Expand Biological Functions

Marc J. Lajoie et al.

SCIENCE (2013)

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Article Gastroenterology & Hepatology

Interleukin-22 Treatment Ameliorates Alcoholic Liver Injury in a Murine Model of Chronic-Binge Ethanol Feeding: Role off Signal Transducer and Activator of Transcription 3

Sung Hwan Ki et al.

HEPATOLOGY (2010)

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Article Gastroenterology & Hepatology

Control of acute, chronic, and constitutive hyperammonemia by wild-type and genetically engineered Lactobacillus plantarum in rodents

Charles Nicaise et al.

HEPATOLOGY (2008)

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Engineering bacteria to modulate host metabolism

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