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Article
Engineering, Biomedical
Kevin M. Beussman et al.
Summary: Measuring cellular traction forces is important for understanding cell behavior and function. A technique called 'black dots' was developed to measure cellular traction forces without altering cell shape or detaching the cells. By evaluating human platelets, the study found that platelets exerting more force have larger spread area, more circular shape, and more uniformly distributed F-actin filaments.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Pingping Han et al.
Summary: The dynamics of cell mechanics and epigenetic signatures play a crucial role in cell behavior and fate, affecting the outcomes of tissue regeneration. While 2D geometric substrates have been widely used to study cell mechanics in response to the extracellular microenvironment, there is limited knowledge about cell mechanobiology and epigenetics in 3D biomaterial matrices. This review explores the potential of additive manufacturing for incorporating multi length-scale geometry features on scaffolds, and discusses how scaffold geometry influences cell and nuclear mechanosensing and epigenetic modifications, ultimately impacting tissue regeneration outcomes.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Alberto Ippolito et al.
Summary: Cell alignment, known as contact guidance, is an important step in the organization of adherent cells. By modeling the response of cells on substrates with alternating soft and stiff stripes, researchers have identified three different mechanisms of cell guidance. Guidance towards stiff stripes is primarily due to molli-avoidance behavior, while decreased collagen density inhibits contact guidance.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Alex Khang et al.
Summary: This study assessed the shape and contractile behaviors of aortic valve interstitial cells using hydrogel-based 3D traction force microscopy. The results showed that changes in cell shape were closely related to the orientation and behavior of stress fibers.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Arina Korneva et al.
Summary: Understanding the responses of astrocytes in the optic nerve head to mechanical and biochemical stimuli is crucial for studying retinal ganglion cell axon degeneration in glaucoma. In a mouse model, elevated intraocular pressure caused alterations in the junctions between astrocytic processes and the peripapillary sclera, leading to increased structural compliance of the optic nerve head. Experimental treatment with TrypLE enzyme partially reproduced these alterations, suggesting the significance of astrocyte-sclera junction separation in increasing ONH compliance.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Jian Sun et al.
Summary: There is increasing evidence that force affects cells and tissues in various physiological and pathological processes, including gene regulation. However, the molecular pathway of force transmission from the nuclear lamina to the chromatin remains unclear. This study reveals that the nuclear protein LAP2 beta mediates force transmission from the nuclear lamina to the chromatin, and this pathway plays a crucial role in gene regulation.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Elrade Rofaani et al.
Summary: This study developed a method to overcome the lack of in vivo-like basement membrane (BM) for in vitro modeling by culturing primordial lung progenitors (PLPs) on an artificial basement membrane (ABM) composed of collagen IV and laminin. The forces generated by the growing epithelial monolayer on the ABM led to geometry-dependent folding and enhanced expression of surfactant protein C and aquaporin 5 compared with a nanofiber-covered substrate. This work emphasizes the importance of recapitulating natural BM for advanced epithelial modeling.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
J. Merson et al.
Summary: The study investigates indentation in network biomaterials and demonstrates a size effect that prevents the use of the Hertz model to infer the elastic constants of the material when the indenter radius is smaller than approximately 12 times the mean segment length of the network. The findings provide guidelines for selecting indentation conditions that ensure the applicability of the Hertz model. Additionally, the method can also be used to estimate the mean segment length of the network based on indentations with different indenter sizes.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Paul Taufalele et al.
Summary: Intratumor heterogeneity plays a crucial role in cancer therapy, and the mechanisms of cell-cell interactions are still unclear. This study investigated the transcriptional differences between tumors with different stiffness using single-cell RNA sequencing. It was found that tumors with higher stiffness had a higher concentration of tumor-promoting macrophages. These findings highlight the importance of cell-cell interactions and macrophage recruitment in tumor development.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Barak Zohar et al.
Summary: Vascularization of 3D engineered tissues is a challenge in tissue engineering. This study introduces the design and fabrication of an AngioTube, a biodegradable macro-vessel surrounded by micro-channel array, to support physiological flow distribution and integration with living capillaries. The engineered micro-channels guide endothelial cells to form patent micro-vessels, and in-vitro and in-vivo models demonstrate anastomosis with vascular networks. This approach offers a new micro-fabrication strategy with high geometrical accuracy at the tissue-scale level.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Joseph Bruns et al.
Summary: Glioblastoma (GBM) spheroid models encapsulated in hydrogels with different stiffness were used to investigate the influence of microenvironment stiffness on drug responsiveness. The results showed higher infiltration ability of spheroids in soft hydrogels, and similar drug response of U87 spheroids in soft and stiff hydrogels, but higher cell viability in the spheroid periphery of stiff hydrogels. Displacement experiments on the stiffness-affected interface further explored the drug responsiveness of the spheroid models.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Chengeng Yang et al.
Summary: Elastin is a crucial protein responsible for the elasticity of various organs. Damage to elastin caused by conditions like hypercalcemia and hyperglycemia can accumulate during aging and aging-associated diseases. This study investigates the structural and elastic effects of hypercalcemia and hyperglycemia on elastin at the molecular level. The findings provide insights into the behavior of tropoelastin and the biomechanics of elastin biomaterials in physiological environments.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Marina Tulchinsky et al.
Summary: Solid-tumor cell invasion often occurs through collective migration of attached cell-cohorts. However, this study reveals that cell-interactions through the substrate can also drive invasiveness. Finite element models and experiments show that close proximity of cells induces additive contributions to indentation depth, especially when unequal forces are applied. These findings suggest the importance of cell-proximity and mechanical factors in driving collective cancer-cell invasiveness.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Emily M. Carvalho et al.
Summary: Biomaterials play a crucial role in studying and controlling cell behaviors, and their viscoelastic properties greatly influence cell behavior. This review discusses the advances in viscoelastic materials for cell engineering, focusing on the mechanisms of collagen, alginate, hyaluronic acid, and fibrous materials in regulating cell behavior.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Jeremy D. Eekhoff et al.
Summary: This study evaluated the quantity, structure, and mechanical contribution of elastin in functionally distinct tendons across species. Tendons with an energy-storing function had more elastin content than tendons with a positional function, and human tendon had at least twice the elastin content of other species. The mechanical effects of elastin degradation varied between tendon type and species, with human tendon and energy-storing tendon being more affected.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Aysegul Dede Eren et al.
Summary: Cells and their surrounding extracellular matrix (ECM) participate in dynamic reciprocity to maintain tissue homeostasis. This involves the deposition of ECM by cells, which then presents the signals that define cell identity. The relationship between cell shape, ECM, and tissue homeostasis is important for understanding pathological conditions and for manipulating cell function in micro-fabricated platforms. This manuscript discusses the loop of phenotype in tendon tissue homeostasis, focusing on cell shape, ECM organization, and signal transduction pathways.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Fan Yang et al.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Dhivya Venugopal et al.
Summary: Electrospun fibrous meshes are widely used for tissue repair due to their ability to guide cell responses. The early innate immune response is crucial for mesh-based tissue regeneration. Matrix designs that don't consider the immune environment can lead to dysregulation and chronic inflammation.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Fernanda F. Fontenele et al.
Summary: In this study, a viscoelastic-plastic constitutive model was developed to analyze the effects of inelasticity and energy dissipation in collagen fibrils, specifically in the context of cyclic loading and overload. The model was validated by comparing the stress-stretch data obtained from experiments with single fibrils subjected to cyclic loading conducted by Liu et al. (2018).
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Stephanie E. Schneider et al.
Summary: Cells are continuously influenced by dynamic environmental cues, especially mechanical cues, which affect their behavior and can lead to morphological and behavioral changes in the nucleus. The nucleus shows a responsive behavior to mechanical forces, propagating from the substrate, and disruption of this behavior can have lasting consequences on cellular functioning. Using an in vitro model of traumatic neural injury, the study demonstrates the dynamic nuclear behavioral response to impulse stretch and highlights the changes in cellular and nuclear behavior caused by mechanical forces.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Roseline Menezes et al.
Summary: GAGs are important components of the extracellular matrix that influence cell behavior and are used in tissue regeneration, biomaterials, and drug delivery applications. This review provides an overview of native GAGs' structure, properties, interaction with proteins, their effect on cell behavior, and mechanical role in the ECM. The use of GAGs in tissue engineering and biomaterial approaches, particularly in orthopedic and wound healing applications, has made significant advances.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Bobby Carroll et al.
Summary: The mechanical properties of tissues are crucial for tissue development and maintenance. By studying the mechanical behavior of fibrous networks embedded with stiff beads, it is found that the presence of beads enhances the mechanical performance of the network under compression, possibly due to the hindrance of network relaxation.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Jyothsna Vasudevan et al.
Summary: The extracellular matrix (ECM) plays a crucial role in the modulation of tumor progression by chemically stimulating tumor cells and offering physical support. The translation between matrix biophysical cues and intracellular signaling has led to rapid growth in cancer mechanobiology. Efforts have been made to develop in vitro tumor mimicking platforms to visualize and quantify mechanical forces within the tissue and understand their impact on tumor cell behavior. Perturbations in these cues can alter cellular mechanisms of mechanotransduction, enhancing malignancy. Engineering techniques can be used to emulate the mechanical properties of tumors and aid in the development of ECM-targeted therapeutics.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Kimberly R. Kroupa et al.
Summary: Recent studies have shown that superficial zone chondrocytes within ar-ticular layers of diarthrodial joints die under normal physiologic loading conditions. In order to understand the mechanical environment that causes their death, a multiscale finite element analysis was conducted to track the changes in interstitial fluid pressure, hydraulic permeability, and volume of SZ chondrocytes under loading. The results indicated that sustained physiological loading can lead to significant loss of intracellular fluid volume, hindering cellular metabolic activity and fluid transport properties.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Ming-Chung Wu et al.
Summary: Through engineered extracellular matrices, the study reveals the association between cell shape, focal adhesion signals, and mesenchymal stromal cell (MSC) lineage commitment. Square cells form mature focal adhesions and symmetrical radial fiber bundles, while circular cells align their transverse fibers parallel to the cell edge. The contractile force generated by the myosin IIA-enriched transverse fibers is distributed through the symmetrical radial fiber bundles, driving focal adhesion organization and maturation and regulating MSC fate decision through YAP mechanotransduction signaling.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Harrison L. Hiraki et al.
Summary: The peritumoral stroma is a complex tissue that influences the migration mode of transformed epithelial cells during metastatic spread. Understanding the role of cues from the stromal microenvironment in regulating migration mode has prognostic and therapeutic value.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Mahvash Jebeli et al.
Summary: Mechanical stress patterns emerging from collective cell behavior play critical roles in morphogenesis, tissue repair, and cancer metastasis. This study aims to investigate the emergence and divergence of heterogeneous cell behaviors over time. The results show that local cell-cell interactions disrupt the predicted global collective cell behavior, leading to heterogeneity in cell behaviors.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Elizabeth Gacek et al.
Summary: This study develops a discrete-continuum modeling scheme for analyzing tissue growth and remodeling, combining microstructural and cellular-scale models with macroscopic finite element models. The model is applied in case studies of arterial media, media and adventitia, and chronic dissection of an arterial wall, providing new data and insights into growth and remodeling processes.
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
Fazlur Rashid et al.
Summary: It has been found that external mechanical forces have an impact on the structure and function of living cells and tissues in both physiological and pathological conditions. However, the mechanisms underlying changes in structures after force cessation are not well understood. This study used single-molecule imaging techniques to demonstrate that local forces applied via integrins cause chromatin decondensation and increased protein mobility in the nucleus, leading to elevated diffusivity of individual protein molecules in the nucleoplasm. The increased protein diffusivity was found to be regulated by nuclear pore complexes, suggesting a mechanomemory effect in the nucleus that persists for tens of minutes after force cessation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Aniket Jana et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Engineering, Biomedical
Orestis G. Andriotis et al.
Summary: Collagen fibrils are the fundamental structural elements in vertebrate animals, providing mechanical support to load-bearing tissues. Extensive research has been conducted to understand the formation and mechanical function of these fibrils, resulting in a wealth of knowledge and new questions. This review examines the current understanding of the mechanical properties of existing fibrils, specifically focusing on their response to tension and the gaps in knowledge that remain to be explored.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Seyed Mohammad Siadat et al.
Summary: Collagen is a unique family of proteins that provide mechanical support to biological tissues. Its formation and remodeling mechanisms are not fully understood, but they play a critical role in vertebrates' survival.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Fan Yang et al.
Summary: This study conducted pure stress relaxation and creep experiments for fully hydrated individual collagen fibrils for the first time, revealing their nonlinear time-dependent behavior consistent with prior studies on macroscale collagenous tissues. This new understanding of the nonlinear viscoelastic behavior of the building blocks of mammalian collagenous tissues may serve as the foundation for improved macroscale tissue models that capture the mechanical behavior across length scales.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Alex Khang et al.
Summary: Cell shape is influenced by mechanical, chemical, and biological mechanisms and reflects the biophysical state of cells. In this study, we used hydrogel-based 3D traction force microscopy to assess the shape and contractile behaviors of aortic valve interstitial cells (AVICs). We observed changes in AVIC geometry over time and found that AVICs were more elongated when relaxed and more spherical in hyper-contraction. These findings provide insights into stress fiber architecture and mechanical behaviors of AVICs.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Wenjie Liu et al.
Summary: This study elucidates the spatiotemporal organization of heterochromatin in the elongated nuclei of cells on aligned nanofibers. The findings suggest that the anisotropy of nuclei is sufficient to drive heterochromatin alterations, and reveal an unconventional H3K9me3 heterochromatin distribution.
Article
Chemistry, Multidisciplinary
Aniket Jana et al.
Summary: This study investigates the plasticity of nucleus morphology in fibrous environments using suspended nanofiber networks. Contrary to cells on 2-dimensional surfaces, cells on fibers display a uniform actin network caging the nucleus, which plays a role in shaping nuclear shapes. The authors found that fiber diameter affects cell contractility and nuclear shape, and is also correlated with the translocation of mechanosensitive transcription factor Yes-associated protein (YAP) to the nucleus.
Review
Cell Biology
Claudia Tanja Mierke
Summary: The viscoelastic response of biological materials, especially the extracellular matrix, is crucial in influencing cellular behavior and the overall shape and function of tissues. However, the impact of viscoelasticity on the functional phenotype of tumor extracellular matrices remains unclear, and ongoing research and development in biophysical technologies are needed to further explore this relationship.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jugroop Singh et al.
Summary: The study shows that rules controlling cell-cell collisions on suspended nanofibers differ significantly from CIL behavior on 2D substrates. Fiber geometry modulates cell behavior, with cell-cell adhesion identified as a key mediator of collision outcomes. The geometry of the fiber can generate entirely new behaviors that cannot be predicted from interactions on flat substrates or micropatterns.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Chemistry, Physical
Farid Alisafaei et al.
Summary: Cells can respond to signals from other cells far away, not only through chemical signals but also through physical signals. Networks of stiff or semiflexible fibers can generate multiple mechanisms for long-range signaling, including force transmission and changes in fiber alignment and density.
Article
Chemistry, Multidisciplinary
Delaram Shakiba et al.
Article
Multidisciplinary Sciences
Bo Ri Seo et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
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Biology
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(2017)
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(2016)
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Sean Meehan et al.
BIOPHYSICAL JOURNAL
(2014)
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Cell Biology
Ayelet Lesman et al.
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(2014)
Review
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Donald E. Ingber et al.
REPORTS ON PROGRESS IN PHYSICS
(2014)
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Ryan J. Petrie et al.
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Cell Biology
Katarina Wolf et al.
JOURNAL OF CELL BIOLOGY
(2013)
Review
Cell Biology
Peter Friedl et al.
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(2012)
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Hisao Ishizuka
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Jeroen Eyckmans et al.
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(2011)
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Stacey A. Maskarinec et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2009)
Review
Cell Biology
S Even-Ram et al.
CURRENT OPINION IN CELL BIOLOGY
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Review
Cell Biology
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DEVELOPMENTAL CELL
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