4.5 Article

Revealing an initiation inhibition of RCA and its application in nucleic acid detection

期刊

ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 55, 期 4, 页码 672-682

出版社

SCIENCE PRESS
DOI: 10.3724/abbs.2023070

关键词

rolling circle amplification; stem; initiation inhibition; design principle; nucleic acid detection

向作者/读者索取更多资源

Rolling circle amplification (RCA) is a widely used biosensing technique. However, the effects of secondary structures on RCA efficiency have not been well studied. This study found that stems in circular templates strongly inhibit RCA, and the primer-stem distance is the cause of this inhibition. Based on these findings, a new nucleic acid detection method was proposed, which has increased sensitivity and convenient visualization. The initiation inhibition of RCA could be helpful for promising detection techniques.
Rolling circle amplification is a widely used biosensing technique. Although various secondary structures have been employed in RCA, their effects on RCA efficiency have seldom been reported. Here, we find that stems in circular templates can strongly inhibit RCA, and the primer-stem distance is responsible for the inhibition. Based on the results, we propose an initiation inhibition mechanism and present a design principle for a general RCA assay. Inspired by this mechanism, we further propose a new nucleic acid detection method. The results verify that this method can increase RCA detection sensitivity according to the target recycling principle. Besides DNA detection, it can also achieve single mismatch discrimination of miRNA detection after optimization. This method also shows convenient visualization detection. The initiation inhibition of RCA could be helpful for RCA applications as promising detection techniques.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据