期刊
ACS NANO
卷 17, 期 10, 页码 9025-9038出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.2c11577
关键词
two-photon polymerization; spatial light modulation; holographic processing; focal adhesion; cell migratory plasticity
In this study, a microclaw-array was fabricated to mimic the extracellular matrix during cancer cell invasion. Different breast epithelial cells exhibited different migration phenotypes on the microclaw-array, depending on their ability to perceive and respond to the topology of the matrix.
The highly aligned extracellular matrix of metastatic breast cancer cells is considered to be the highway of cancer invasion, which strongly promotes the directional migration of cancer cells to break through the basement membrane. However, how the reorganized extracellular matrix regulates cancer cell migration remains unknown. Here, a single exposure of a femtosecond Airy beam followed by a capillary -assisted self-assembly process was used to fabricate a micro -claw-array, which was used to mimic the highly oriented extracellular matrix of tumor cells and the pores in the matrix or basement membrane during cell invasion. Through the experiment, we found that metastatic breast cancer MDA-MB-231 cells and normal breast epithelial MCF-10A cells exhibit three major migration phenotypes on microclaw-array assembled with different lateral spacings: guidance, impasse, and penetration, whereas guided and penetrating migration are almost completely arrested in noninvasive MCF-7 cells. In addition, different mammary breast epithelial cells differ in their ability to spontaneously perceive and respond to the topology of the extracellular matrix at the subcellular and molecular levels, which ultimately affects the cell migratory phenotype and pathfinding. Altogether, we fabricated a microclaw-array as a flexible and high-throughput tool to mimic the extracellular matrix during invasion to study the migratory plasticity of cancer cells.
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