4.6 Article

Characterization of Unexpected Self-Acylation Activity of Acyl Carrier Proteins in a Modular Type I Apicomplexan Polyketide Synthase

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Multidisciplinary Sciences

A single amino acid residue controls acyltransferase activity in a polyketide synthase from Toxoplasma gondii

Hannah K. D'Ambrosio et al.

Summary: We reveal the sequences of three acyltransferase domains from a polyketide synthase in Toxoplasma gondii and find them distinct from domains in well-characterized microbial biosynthetic gene clusters. Biochemical investigations show that two domains, AT1 and AT2, hydrolyze malonyl-CoA while the terminal domain, AT3, is non-functional. Furthermore, we identify an on-off switch residue that controls activity, with a single amino acid change in AT3 conferring hydrolysis activity and the analogous mutation in AT2 eliminating activity. This study lays the foundation for further molecular and structural studies on polyketide synthases from T. gondii and other protists.

ISCIENCE (2022)

Review Biochemistry & Molecular Biology

Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways

Ariana Sulpizio et al.

Summary: Type II polyketide synthases (PKSs) are protein assemblies that manufacture complex molecules with the help of acyl carrier proteins (ACPs). Understanding the dynamics and interactions of ACPs is crucial for redesigning type II PKSs. By utilizing chemical probes and molecular dynamic simulations, insights into the structure and function of type II PKS ACPs can be gained, potentially unlocking new biosynthetic potential.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Chemistry, Physical

Transacylation Kinetics in Fatty Acid and Polyketide Synthases and its Sensitivity to Point Mutations**

Franziska Stegemann et al.

Summary: This study investigates the mechanisms of FASs and PKSs in synthesizing important products and the substrate selectivity and enzymatic properties of different ATs. Findings reveal that ATs in PKS systems occurring in bacteria are slower than ATs in mammalian FASs, reflecting the respective purposes and metabolic contexts within the organism.

CHEMCATCHEM (2021)

Review Biotechnology & Applied Microbiology

Natural products in drug discovery: advances and opportunities

Atanas G. Atanasov et al.

Summary: Natural products and their analogues have historically played a significant role in pharmacotherapy, however, they also present challenges. Recent technological and scientific developments are addressing these challenges and revitalizing interest in natural products as drug leads, particularly for combating antimicrobial resistance.

NATURE REVIEWS DRUG DISCOVERY (2021)

Review Plant Sciences

An Overview of the Medicinally Important Plant Type III PKS Derived Polyketides

Renu Bisht et al.

Summary: Plants produce a variety of secondary metabolites through type III PKSs, which have pharmaceutical, medicinal, and industrial applications, making them important tools for bioengineering. These secondary metabolites affect human health and provide survival advantages for the plant species, showcasing the potential for generating new drugs and modified products through enzyme manipulation.

FRONTIERS IN PLANT SCIENCE (2021)

Article Biotechnology & Applied Microbiology

Engineering the acyltransferase domain of epothilone polyketide synthase to alter the substrate specificity

Huimin Wang et al.

Summary: The study found that substitution and deletion of catalytic domains can effectively produce desired compounds, and the selection of linkers is crucial for maintaining intact PKS catalytic activity.

MICROBIAL CELL FACTORIES (2021)

Article Biochemistry & Molecular Biology

Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation

Ivica Letunic et al.

Summary: The Interactive Tree Of Life is an online tool for displaying, manipulating, and annotating phylogenetic and other trees. The latest version 5 introduces a new tree display engine and several new features, such as a new dataset type and expanded annotation options. Users can now also manually annotate trees by hand, providing more flexibility and control.

NUCLEIC ACIDS RESEARCH (2021)

Article Biochemistry & Molecular Biology

antiSMASH 6.0: improving cluster detection and comparison capabilities

Kai Blin et al.

Summary: antiSMASH, a tool for detecting biosynthetic gene clusters in microorganisms, has been updated to version 6, which increases the supported cluster types, displays the structure of multi-modular BGCs, adds a new comparison algorithm, allows integration of results from other prediction tools, and improves detection of tailoring enzymes in RiPP clusters.

NUCLEIC ACIDS RESEARCH (2021)

Article Multidisciplinary Sciences

Highly accurate protein structure prediction with AlphaFold

John Jumper et al.

Summary: Proteins are essential for life, and accurate prediction of their structures is a crucial research problem. Current experimental methods are time-consuming, highlighting the need for accurate computational approaches to address the gap in structural coverage. Despite recent progress, existing methods fall short of atomic accuracy in protein structure prediction.

NATURE (2021)

Article Chemistry, Multidisciplinary

Solution Structure and Conformational Flexibility of a Polyketide Synthase Module

Maja Klaus et al.

Summary: Polyketide synthases (PKSs) are versatile enzymes widely distributed in bacteria and fungi, playing a crucial role in the synthesis of important drugs. However, the lack of understanding about key structural properties poses a challenge for custom compound synthesis. This study characterizes the structure and dynamics of PKS modules, revealing that substrate shuttling may be guided by a nonspecific electrostatic interaction network.

JACS AU (2021)

Review Plant Sciences

Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019

David J. Newman et al.

JOURNAL OF NATURAL PRODUCTS (2020)

Article Chemistry, Multidisciplinary

Complete Reconstitution and Deorphanization of the 3 MDa Nocardiosis-Associated Polyketide Synthase

Kai P. Yuet et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Review Biochemistry & Molecular Biology

Repurposing Modular Polyketide Synthases and Non-ribosomal Peptide Synthetases for Novel Chemical Biosynthesis

Soonkyu Hwang et al.

FRONTIERS IN MOLECULAR BIOSCIENCES (2020)

Article Multidisciplinary Sciences

Structure, function and dynamics in acyl carrier proteins

Rohit Farmer et al.

PLOS ONE (2019)

Review Chemistry, Multidisciplinary

Evolution and Diversity of Assembly-Line Polyketide Synthases

Aleksandra Nivina et al.

CHEMICAL REVIEWS (2019)

Review Biochemical Research Methods

Engineering modular polyketide synthases for production of biofuels and industrial chemicals

Wenlong Cai et al.

CURRENT OPINION IN BIOTECHNOLOGY (2018)

Article Multidisciplinary Sciences

Deciphering the Draft Genome of Toxoplasma gondii RH Strain

Yee-Ling Lau et al.

PLOS ONE (2016)

Article Multidisciplinary Sciences

Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis

Akimasa Miyanaga et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

Article Biochemistry & Molecular Biology

Assembly Line Polyketide Synthases: Mechanistic Insights and Unsolved Problems

Chaitan Khosla et al.

BIOCHEMISTRY (2014)

Article Biochemistry & Molecular Biology

Mechanism and Specificity of an Acyltransferase Domain from a Modular Polyketide Synthase

Briana J. Dunn et al.

BIOCHEMISTRY (2013)

Article Biochemistry & Molecular Biology

Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

Fabian Sievers et al.

MOLECULAR SYSTEMS BIOLOGY (2011)

Review Biochemistry & Molecular Biology

Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase

David E. Cane

JOURNAL OF BIOLOGICAL CHEMISTRY (2010)

Article Multidisciplinary Sciences

Molecular recognition between ketosynthase and acyl carrier protein domains of the 6-deoxyerythronolide B synthase

Shiven Kapur et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)

Article Biochemistry & Molecular Biology

Conformational flexibility of metazoan fatty acid synthase enables catalysis

Edward J. Brignole et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2009)

Article Biochemistry & Molecular Biology

Self-acylation properties of type II fatty acid biosynthesis acyl carrier protein

Ashish Misra et al.

CHEMISTRY & BIOLOGY (2007)

Review Biochemistry & Molecular Biology

The ubiquitous carrier protein - a window to metabolite biosynthesis

Andrew C. Mercer et al.

NATURAL PRODUCT REPORTS (2007)

Article Biochemical Research Methods

A continuous coupled enzyme assay for bacterial malonyl-CoA:acyl carrier protein transacylase (FabD)

J Molnos et al.

ANALYTICAL BIOCHEMISTRY (2003)

Article Multidisciplinary Sciences

Biosynthesis of complex polyketides in a metabolically engineered strain of E-coli

BA Pfeifer et al.

SCIENCE (2001)