期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 37, 期 4, 页码 1379-1389出版社
KARGER
DOI: 10.1159/000430403
关键词
Dachengqi decoction; Severe acute pancreatitis; HMGB1; TLRs; NF-kappa B signaling pathway; MAPK signaling pathway
资金
- Putuo District Health System Innovation Research Fund [2012PTKW003]
- Inovation Team of Shanghai Traditional Chinese Medicine Training Scheme of Back-up Experts of Shanghai University of Traditional Chinese Medicine
- Xinglin New Star Program [ZY3-RCPY-2-2072]
Background/Aims: Severe acute pancreatitis (SAP) is a sudden inflammation of the pancreas. The traditional Chinese medicine formula Dachengqi decoction (DCQD) is proven to be beneficial in the comprehensive treatment for pancreatitis patients in clinical practice. However, the molecular mechanism of DCQD on SAP remains unclear. High mobility group box 1(HMGB1) that functions as a damage-associated molecular pattern molecule (DAMP) has attracted much interest. Methods: In this study, we used lipopolysaccharide ([PS) and cerulein to induce severe acute pancreatitis in C57BL/6 mice with subsequent administration with low, medium and high dose (2.3 g/kg, 7 g/kg and 21 g/kg, respectively) of DCQD. Results: DCQD treatment improved the pathological score and decreased serum amylase and lipase in a dose-dependent manner. In addition, it suppressed the immune cell-induced secretion of HMGB1 and its translocation from the nucleus to the cytoplasm, thus repressing the expression of IL-6 and TNF-alpha. Further, pretreatment with DCQD decreased responses of TLRs, and suppressed the activation of NF-kappa B and p38 MAPK pathway. Conclusion: Decreasing the secretion of HMGB1 could reduce pro-inflammatory cytokines, which may help cutting down the risks of development from localized pathological changes to a systemic inflammatory response syndrome and even lead to multiple organ failure. (C) 2015 The Author(s) Published by S. Karger AG, Basel
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