4.6 Review

Biological nitrogen fixation in cereal crops Progress, strategies, and perspectives

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Review Microbiology

Future Outlook of Transferring Biological Nitrogen Fixation (BNF) to Cereals and Challenges to Retard Achieving this Dream

Abdelaal Shamseldin

Summary: BNF is a vital biological process that enables cereals to fix atmospheric nitrogen gas through the symbiosis of bacteria and nitrogenase. While some progress has been made by scientists, there are still challenges that need to be addressed.

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Summary: The review focuses on the progress in understanding the evolution and differences between mycorrhizal and root nodule symbioses in plants. It highlights the importance of plants being able to distinguish between beneficial symbionts and potential pathogens for their survival. The overlap in signaling pathways and infection components of these symbioses reflects their evolutionary relatedness, while the different outputs, phosphate uptake versus N fixation, require fundamentally different components and regulators.

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Engineered plant control of associative nitrogen fixation

Timothy L. Haskett et al.

Summary: Engineering N-2-fixing symbioses between cereals and diazotrophic bacteria represents a promising strategy to sustainably deliver biologically fixed nitrogen in agriculture. The development of a homozygous rhizopine producing barley line and a hybrid rhizopine uptake system significantly improved the sensitivity for rhizopine perception in the bacterium Azorhizobium caulinodans ORS571. This work represents a key milestone toward the development of a synthetic plant-controlled symbiosis.

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Bacillus subtilis HG-15, a Halotolerant Rhizoplane Bacterium, Promotes Growth and Salinity Tolerance in Wheat (Triticum aestivum)

Chao Ji et al.

Summary: Certain plant growth-promoting bacteria can alleviate salt stress damage in plants. Bacillus subtilis HG-15, a halotolerant bacterium, shows potential as a microbial inoculant to protect wheat under salt stress conditions by improving growth and inducing systemic tolerance.

BIOMED RESEARCH INTERNATIONAL (2022)

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Genetic modification of flavone biosynthesis in rice enhances biofilm formation of soil diazotrophic bacteria and biological nitrogen fixation

Dawei Yan et al.

Summary: This study describes a novel approach to enhance biological nitrogen fixation (BNF) in rice plants by increasing the production of compounds that stimulate biofilm formation in soil diazotrophic bacteria. The modified rice plants displayed improved BNF, leading to increased grain yield under limiting soil nitrogen conditions. Manipulation of the flavone biosynthetic pathway shows promise as a feasible strategy for inducing BNF in cereals and reducing the dependence on inorganic nitrogen fertilizers.

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Functional Nitrogenase Cofactor Maturase NifB in Mitochondria and Chloroplasts of Nicotiana benthamiana

Xi Jiang et al.

Summary: Biological nitrogen fixation is the process of converting inert atmospheric nitrogen gas into reactive ammonia, which can reduce the dependency on nitrogen fertilizers in plants by introducing functional nitrogenase. NifB is a critical protein in nitrogen fixation and can be restored by expressing nifB genes from different sources. Accumulation of functional NifB proteins in chloroplasts and mitochondria is crucial for engineering biological nitrogen fixation in plants.
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A highly conserved core bacterial microbiota with nitrogen-fixation capacity inhabits the xylem sap in maize plants

Liyu Zhang et al.

Summary: The study characterizes the microbiota in the xylem of maize plants and finds that bacteria carrying nitrogen fixing genes provide the host plant with nitrogen. By using synthetic communities, the authors confirm the role of xylem inhabiting and nitrogen fixing bacteria in nitrogen supply to the host plant. The study highlights the importance of the xylem microbiota in crop performance.

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GWAS, MWAS and mGWAS provide insights into precision agriculture based on genotype-dependent microbial effects in foxtail millet

Yayu Wang et al.

Summary: In this study, the associations between genotypic, phenotypic, and rhizoplane microbiota variables of foxtail millet are investigated. A total of 257 rhizoplane microbial biomarkers associated with key agronomic traits are identified. The composition of the rhizoplane microbiota is mainly driven by variations in plant genes related to immunity, metabolites, hormone signaling and nutrient uptake. The microbial-mediated growth effects on foxtail millet are dependent on the host genotype.

NATURE COMMUNICATIONS (2022)

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Nitrogenase Cofactor Maturase NifB Isolated from Transgenic Rice is Active in FeMo-co Synthesis

Wenshu He et al.

Summary: This study shows that expression and purification of NifB protein from archaea in transgenic rice plants can successfully produce soluble proteins in plants. This is an important breakthrough for achieving independent biological nitrogen fixation in cereals.

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An SHR-SCR module specifies legume cortical cell fate to enable nodulation

Wentao Dong et al.

Summary: Legumes have a unique stem cell program in cortical cells that specifies their fate and responds to rhizobial signals, initiating legume-specific cortical cell division. This SHR-SCR network is conserved across legume species and plays a crucial role in the evolution of rhizobial endosymbiosis.

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Plant-Microbe Interaction: Aboveground to Belowground, from the Good to the Bad

Kalaivani Nadarajah et al.

Summary: Research on the soil rhizosphere region is crucial in identifying ways to restore soil health and fertility, with microbes playing key roles in plant growth, disease suppression, and nutrient cycling. Identifying the specific functions of these microbes can aid in designing sustainable agricultural practices.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Review Plant Sciences

Leguminous nodule symbiosis involves recruitment of factors contributing to lateral root development

Takashi Soyano et al.

Summary: The formation of root nodules in legumes and actinorhizal plants involves distinct processes compared to lateral root development, with different types of nodules showing variation. Evolution of new organs like root nodules is believed to occur through rearrangement of molecular networks guided by neo-functionalized factors. Evidence suggests that root nodule formation is linked to root or lateral root developmental pathways acquired by the common ancestor of nitrogen-fixing Glade.

CURRENT OPINION IN PLANT BIOLOGY (2021)

Article Multidisciplinary Sciences

NIN-like protein transcription factors regulate leghemoglobin genes in legume nodules

Suyu Jiang et al.

Summary: Leghemoglobins play a crucial role in facilitating nitrogen fixation in legume nodules by maintaining a micro-oxic environment while channeling oxygen for bacterial respiration. The NLP transcription factors NLP2 and NIN are found to directly activate leghemoglobin expression through a specific promoter motif, and knockout of the NRE-like element significantly decreases leghemoglobin expression. These findings suggest that the NLP-leghemoglobin module for oxygen buffering in nodules has ancient origins dating back to the pairing of NLPs with nonsymbiotic hemoglobins in hypoxic conditions.

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Review Agronomy

Nitrogen fixation in maize: breeding opportunities

Seema Sheoran et al.

Summary: Maize is a highly versatile crop with a huge demand for nitrogen, and the development of biological nitrogen fixation in cereals is seen as a sustainable solution to reduce reliance on synthetic fertilizers. Researchers have identified a large number of endophytic diazotrophs associated with maize, and investigating their genetic and molecular interactions can pave the way for introducing nitrogen fixation in maize breeding. Through advanced understanding and breeding techniques, researchers are on the verge of engineering symbiotic relationships in cereals to improve sustainable production systems and achieve food security.

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A Model for Nitrogen Fixation in Cereal Crops

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A shared gene drives lateral root development and root nodule symbiosis pathways in Lotus

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Stefan Buren et al.

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Expression of 16 Nitrogenase Proteins within the Plant Mitochondrial Matrix

Robert S. Allen et al.

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Symbiotic Nitrogen Fixation and the Challenges to Its Extension to Nonlegumes

Florence Mus et al.

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Raoultella sp strain L03 fixes N2 in association with micropropagated sugarcane plants

Ting Luo et al.

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Using synthetic biology to increase nitrogenase activity

Xin-Xin Li et al.

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Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast

Gema Lopez-Torrejon et al.

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Effects of Selected Diazotrophs on Maize Growth

Medhin H. Kifle et al.

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Robust biological nitrogen fixation in a model grass-bacterial association

Vania C. S. Pankievicz et al.

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Structure, variation, and assembly of the root-associated microbiomes of rice

Joseph Edwards et al.

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Biotechnological solutions to the nitrogen problem

Giles E. D. Oldroyd et al.

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Synthetic biology approaches to engineering the nitrogen symbiosis in cereals

Christian Rogers et al.

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Reconstruction and minimal gene requirements for the alternative iron-only nitrogenase in Escherichia coli

Jianguo Yang et al.

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