4.3 Article

Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics

期刊

BIOENGINEERING-BASEL
卷 9, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/bioengineering9100567

关键词

atomic force microscopy; single-cell spectroscopy; bacterial adhesion; cell surface; cell respiration; dental implant

资金

  1. Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under the Collaborative Research Center [SFB/TRR-298-SIIRI, 426335750]
  2. Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy (DFG Cluster of Excellence) [EXC 2177/1-Hearing4All, 390895286]

向作者/读者索取更多资源

This study provides insights into the initial adhesion of oral bacteria and supports the development of infection-resistant implant materials in the future.
Bacterial adhesion to dental implants is the onset for the development of pathological biofilms. Reliable characterization of this initial process is the basis towards the development of anti-biofilm strategies. In the present study, single-cell force spectroscopy (SCFS), by means of an atomic force microscope connected to a microfluidic pressure control system (FluidFM), was used to comparably measure adhesion forces of different oral bacteria within a similar experimental setup to the common implant material titanium. The bacteria selected belong to different ecological niches in oral biofilms: the commensal pioneers Streptococcus oralis and Actinomyces naeslundii; secondary colonizer Veillonella dispar; and the late colonizing pathogens Porphyromonas gingivalis as well as fimbriated and non-fimbriated Aggregatibacter actinomycetemcomitans. The results showed highest values for early colonizing pioneer species, strengthening the link between adhesion forces and bacteria's role in oral biofilm development. Additionally, the correlation between biophysical cellular characteristics and SCFS results across species was analyzed. Here, distinct correlations between electrostatically driven maximum adhesion force, bacterial surface elasticity and surface charge as well as single-molecule attachment points, stretching capability and metabolic activity, could be identified. Therefore, this study provides a step towards the detailed understanding of oral bacteria initial adhesion and could support the development of infection-resistant implant materials in future.

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