期刊
CELLULAR MICROBIOLOGY
卷 18, 期 2, 页码 195-210出版社
WILEY
DOI: 10.1111/cmi.12495
关键词
-
资金
- University of Burgundy
- Hopital du Bocage, Dijon, France
- Agence Nationale de la Recherche (KANJI) [ANR-08-MIEN- 033-01]
- BIOASTER-Sanofi-Alliance pour les Sciences de la Vie et de la Sante (AVIESAN) joint program (Candida) [BAP110]
- French Government through the Investissement d'Avenir program [ANR-10-AIRT-03]
Candida albicans is the most frequent yeast responsible for systemic infections in humans. These infections mainly originate from the gastrointestinal tract where C. albicans can invade the gut epithelial barrier to gain access to the bloodstream. Along the gut, pathogens can useMicrofold (M) cells as a portal of entry to cross the epithelial barrier. M cells are specialized cells mainly located in the follicule-associated epithelium of Peyer patches. In this study, we used scanning electron and fluorescence microscopy, adhesion and invasion assays and fungal mutants to investigate the interactions of C. albicans with M cells obtained in an established in vitro model whereby enterocyte-like Caco-2 cells co-cultured with the Raji B cell line undergo a phenotypic switch to morphologically and functionally resembling M cells. Our data demonstrate that C. albicans colocalizes with and invades preferentially M cells, providing evidence that the fungus can use M cells as a portal of entry into the intestinal barrier. In addition to active penetration, F-actin dependent endocytosis contributes to internalization of the fungus into M cells through a mechanism involving hypha-associated invasins including Ssa1 and Als3.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据