Automatic Calculation of the Kinetic Parameters of Enzymatic Reactions with Their Standard Errors Using Microsoft Excel br

We created a Microsoft Excel file, Enzyme Kinetics_Calculator, which includes macro pro- grams that automatically calculates kinetic parameters for typical kinetic equations of enzymatic reac- tions, accompanied by their standard errors, by minimizing the residual sum of squares thereof. The [S]-v plot is automatically drawn with the theoretical lines and, similarly, the 1/[S]-1/v plot in the case of linear theoretical lines. Enzyme Kinetics_Calculator is available as a supplementary file for this paper (see J. Appl. Glycosci. Web site).

Automated summary

We developed a Microsoft Excel file called Enzyme Kinetics_Calculator, which contains macro programs that can automatically calculate kinetic parameters and their standard errors for typical enzymatic reactions. The file also includes functionalities to generate [S]-v plots and 1/[S]-1/v plots with theoretical lines. Enzyme Kinetics_Calculator is available as a supplementary file on the J. Appl. Glycosci. website.