期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 72, 期 9, 页码 1779-1794出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-015-1836-z
关键词
microRNA; 3 ' UTR; RNA secondary structure; Luciferase assay; AntagomiRs; BDNF
资金
- Academy of Finland [136591, 140983, 263700]
- Institute of Biotechnology
- Doctoral Program Brain Mind
- Finnish Graduate School of Neurosciences
- Academy of Finland (AKA) [140983, 263700, 140983, 136591, 136591, 263700] Funding Source: Academy of Finland (AKA)
During the past decade, the identification of microRNA (miR) targets has become common laboratory practice, and various strategies are now used to detect interactions between miRs and their mRNA targets. However, the current lack of a standardized identification process often leads to incomplete and/or conflicting results. Here, we review the problems most commonly encountered when verifying miR-mRNA interactions, and we propose a workflow for future studies. To illustrate the challenges faced when validating a miR target, we discuss studies in which the regulation of brain-derived neurotrophic factor by miRs was investigated, and we highlight several controversies that emerged from these studies. Finally, we discuss the therapeutic use of miR inhibitors, and we discuss several questions that should be addressed before proceeding to preclinical testing.
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