期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 194, 期 3, 页码 423-433出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2016.04.007
关键词
Electron microscopy density map; Single particle analysis; Denoising; Macromolecular complexes; Structure; Gaussian functions
资金
- CNRS (France)
- CSIC (Spain) [Projet International de Cooperation Scientifique - PICS]
- French National Research Agency ANR [ANR-11-BSV8-010-04]
- Spanish Ministry of Economy and Competitiveness [AIC-A-2011-0638, BIO2013-44647-R]
- Comunidad de Madrid [CAM S2010/BMD-2305]
- European Social Fund
- Ministerio de Educacion y Ciencia (Ramon y Cajal fellowship)
Cryo-electron microscopy (cryo-EM) of frozen-hydrated preparations of isolated macromolecular complexes is the method of choice to obtain the structure of complexes that cannot be easily studied by other experimental methods due to their flexibility or large size. An increasing number of macromolecular structures are currently being obtained at subnanometer resolution but the interpretation of structural details in such EM-derived maps is often difficult because of noise at these high-frequency signal components that reduces their contrast. In this paper, we show that the method for EM density-map approximation using Gaussian functions can be used for denoising of single-particle EM maps of high (typically subnanometer) resolution. We show its denoising performance using simulated and experimental EM density maps of several complexes. (C) 2016 Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据