期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 195, 期 1, 页码 93-99出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2016.04.010
关键词
Electron microscopy; Single particle analysis; Image processing; Software package; Workflows; Reproducibility
资金
- Fundacion General CSIC
- Spanish Ministry of Economy and Competitiveness (MINECO)
- MINECO [AIC-A-2011-0638, BFU2013-41249-P, BIO2013-44647-R]
- Comunidad de Madrid through Grant CAM [S2010/BMD-2305]
- European Union (EU)
- Horizon [EINFRA-2014-2, 654142, EINFRA-2015-1, 675858]
- Instruct, a component of the European Strategy Forum on Research Infrastructures (ESFRI)
In the past few years, 3D electron microscopy (3DEM) has undergone a revolution in instrumentation and methodology. One of the central players in this wide-reaching change is the continuous development of image processing software. Here we present Scipion, a software framework for integrating several 3DEM software packages through a workflow-based approach. Scipion allows the execution of reusable, standardized, traceable and reproducible image-processing protocols. These protocols incorporate tools from different programs while providing full interoperability among them. Scipion is an open-source project that can be downloaded from http://scipion.cnb.csic.es. (C) 2016 Elsevier Inc. All rights reserved.
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