期刊
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 162, 期 -, 页码 4-26出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jsbmb.2015.11.017
关键词
Sterol; Cholesterol; Hydroxycholesterol; Epoxycholesterol; Liquid chromatography; Mass spectrometry; Derivatisation
资金
- UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/I001735/1, BB/L001942/1]
- BBSRC [BB/I001735/1, BB/L001942/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L001942/1, BB/I001735/1] Funding Source: researchfish
Oxysterols are oxygenated forms of cholesterol or its precursors. They are formed enzymatically and via reactive oxygen species. Oxysterols are intermediates in bile acid and steroid hormone biosynthetic pathways and are also bioactive molecules in their own right, being ligands to nuclear receptors and also regulators of the processing of steroid regulatory element-binding proteins (SREBPs) to their active forms as transcription factors regulating cholesterol and fatty acid biosynthesis. Oxysterols are implicated in the pathogenesis of multiple disease states ranging from atherosclerosis and cancer to multiple sclerosis and other neurodegenerative diseases including Alzheimer's and Parkinson's disease. Analysis of oxysterols is challenging on account of their low abundance in biological systems in comparison to cholesterol, and due to the propensity of cholesterol to undergo oxidation in air to generate oxysterols with the same structures as those present endogenously. In this article we review the mass spectrometry based methods for oxysterol analysis paying particular attention to analysis by liquid chromatography mass spectrometry (LC-MS). (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license.
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