期刊
CELL STEM CELL
卷 17, 期 6, 页码 758-766出版社
CELL PRESS
DOI: 10.1016/j.stem.2015.10.001
关键词
-
资金
- HHMI
- Bio & Medical Technology Development Program of NRF and MSIP of Republic of Korea [2012M3A9C6049723, 2015M3A9C6028961]
- Cha Medical Foundation in Republic of Korea
- Japan Society for the Promotion of Science (JSPS)
- Juvenile Diabetes Research Foundation [3-2012-293]
- National Research Foundation of Korea [22A20130012640, 2015M3A9C6028961, 2012M3A9C6049723] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The extremely low efficiency of human embryonic stem cell (hESC) derivation using somatic cell nuclear transfer (SCNT) limits its potential application. Blastocyst formation from human SCNT embryos occurs at a low rate and with only some oocyte donors. We previously showed in mice that reduction of histone H3 lysine 9 trimethylation (H3K9me3) through ectopic expression of the H3K9me3 demethylase Kdm4d greatly improves SCNT embryo development. Here we show that overexpression of a related H3K9me3 demethylase KDM4A improves human SCNT, and that, as in mice, H3K9me3 in the human somatic cell genome is an SCNT reprogramming barrier. Overexpression of KDM4A significantly improves the blastocyst formation rate in human SCNT embryos by facilitating transcriptional reprogramming, allowing efficient derivation of SCNT-derived ESCs using adult Age-related Macular Degeneration (AMD) patient somatic nuclei donors. This conserved mechanistic insight has potential applications for improving SCNT in a variety of contexts, including regenerative medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据