Bioactive protein hydrolysates obtained from amaranth by fermentation with lactic acid bacteria and Bacillus species

Protein hydrolysates are a promising source of bioactive peptides. One strategy by which they can be obtained is fermentation. This method uses the proteolytic system of microorganisms to hy-drolyze the parental protein. Fermentation is a little-explored method for obtaining protein hy-drolysates from amaranth. Different strains of lactic acid bacteria (LAB) and Bacillus species isolated from goat milk, broccoli, aguamiel, and amaranth flour were used in this work. First, the total protein degradation (%TPD) of amaranth demonstrated by the strains was determined. The results ranged from 0 to 95.95%, the strains that produced a higher %TPD were selected. These strains were identified by molecular biology and were found to correspond to the genera Enterococcus, Lactobacillus, Bacillus, and Leuconostoc. Fermentation was carried out with amaranth flour and the selected strains. After this process, water/salt extracts (WSE) containing the released protein hydrolysates were obtained from amaranth doughs. The peptide concentration was measured by the OPA method. The antioxidant, antihypertensive and antimicrobial activity of the WSE was evaluated. In the FRAP test, the best WSE was LR9 with a concentration of 1.99 mu MTE/L +/- 0.07. In ABTS, 18C6 obtained the highest concentration with 19.18 mu MTE/L +/- 0.96. In the DPPH test, there was no significant difference. In terms of antihypertensive activity, inhibition percentages ranging from 0 to 80.65% were obtained. Some WSE were found to have antimi-crobial properties against Salmonella enterica and Listeria monocytogenes. Fermentation of amaranth with LAB and Bacillus spp. allowed the release of protein hydrolysates with antioxidant, antihypertensive, and antimicrobial activity.

Automated summary

Protein hydrolysates, obtained through fermentation, have shown promise as a source of bioactive peptides. In this study, lactic acid bacteria and Bacillus strains isolated from various sources were used to ferment amaranth flour and obtain protein hydrolysates. The resulting water/salt extracts were found to possess antioxidant, antihypertensive, and antimicrobial activity.