Microbial profile of T-shirts after a fitness session of Chinese students

Clothing textiles could protect our human skin against external factors, but the microbial population, including conditional pathogens, in clothing, would cause unpleasant odor, Skin inflammation, and textile deterioration. Several studies have reported that microbiomes on clothes are affected by skin microorganisms of individuals, the local environment and the types of textile fabrics, but little is known about how the textile microbial community is shaped in the Chinese population. In this study, 10 healthy young students were recruited to successfully wear the T-shirts made with 3 different fabrics (polyester, cotton, and blending fabrics of polyester and cotton) during physical exercise. Total deoxyribonucleic acid (DNA) was extracted from 30 T-shirts and 16s rRNA gene amplicon sequencing was applied to estimate the absolute abundances of bacteria in the samples. The main bacteria on wore T-shirts were Staphylococcus (21.66%) Enhydrobacter (13.81%), Pantoea (8.14%), Acinetobacter (7.81%), Pseudomonas (6.18%), Cutibacterium (4.99%). However, no difference of alpha and beta diversity was observed among the three textile fabrics. Further analysis found that Pantoea and Pseudomonas, mainly from the environment, enriched on the polyester, but not on cotton, while Enhydrobacter, from human skin, has the growth advantage on cotton, and the blending fabric in between. Collectively, our study preliminary explored the clothes microbiome in Chinese young students, contributing to helping understand the role of clothing microorganisms on human health.

Automated summary

This study explored the clothing microbiome in Chinese young students, revealing the impact of individual skin microbiota, environmental factors, and textile fabric types on clothing microorganisms. The diversity of clothing microbial communities was not influenced by different fabric types, but environmental bacteria Pantoea and Pseudomonas enriched on polyester, while skin-derived Enhydrobacter had a growth advantage on cotton.