期刊
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
卷 7, 期 4, 页码 1167-1172出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.synbio.2022.08.004
关键词
Oxidosqualene cyclase; Zea mays; Hop-17(21)-en-3-ol; Hopenol B; Simiarenol
资金
- National Key Research and Development Program of China [2020YFA0907900, 2019YFA0905700]
- National Science Resource Investigation Pro-gram of China [2019FY100100]
- international Partnership Program of Chinese Academy of Sciences [153D31KYSB20170121]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27020206]
- Strategic Biological Resources Service Network Plan of the Chinese Academy of Sciences [KFJ-BRP-009, KFJ-BRP-017-60]
In this study, a novel oxidosqualene cyclase (OSC) enzyme was identified and characterized from maize for the first time. The enzyme was found to catalyze the biosynthesis of three bioactive terpenoids, laying the foundation for the synthetic biology production of these valuable compounds.
Zea mays (maize) is an important agricultural crop that produces a variety of valuable terpenoids, including several triterpenoids. However, no oxidosqualene cyclase (OSC) enzymes, which catalyze the first step in tri-terpenoid biosynthesis, have been identified in maize. Here, we identified a novel OSC (ZmOSC1) in maize using a combination of genomic mining and phylogenetic analyses. To functionally characterize the candidate OSC, we constructed a yeast strain that produced high levels of 2,3-oxidosqualene. When ZmOSC1 was expressed in this strain, three compounds were detected and identified as hop-17(21)-en-3-ol, hopenol B and simiarenol, respectively. For their biosynthesis, we proposed a potential cyclization mechanism catalyzed by ZmOSC1 via the generation of a dammarenyl cation, followed by sequential cationic ring expansion, cyclization, cationic migration and further proton elimination. This study discovered and characterized an OSC from maize for the first time, and laid a foundation to produce three bioactive pentacyclic triterpenes, hop-17(21)-en-3-ol, hopenol B and simiarenol, using synthetic biology approaches.
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