期刊
ACS APPLIED NANO MATERIALS
卷 6, 期 3, 页码 2249-2257出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsanm.2c05601
关键词
DNA− ligand complexes; DNA structure; structural transitions; mechanical nanoresonator; nano-biosensing
Real-time monitoring of structural transitions in DNA complexes is currently limited to complex techniques and chemically modified oligonucleotides. Here, we show that nanomechanical resonators made of different DNA complexes can be used to monitor structural variations by tracking the flexural resonance frequency as a function of temperature. This has implications in environmental studies and in vitro experiments for evaluating the effects of drugs on DNA.
Despite being widely recognized as of paramount importance in molecular biology, real-time monitoring of structural transitions in DNA complexes is currently limited to complex techniques and chemically modified oligonucleotides. Here, we show that nanomechanical resonators made of different DNA complexes, such as pristine dsDNA, ssDNA, and DNA intercalated with dye molecules or chemotherapeutic agents, are characterized by unique fingerprint curves when their flexural resonance frequency is tracked as a function of temperature. Such frequency shifts can be successfully used to monitor structural variations in DNA complexes, such as B-to-A form and helix-to-coil transitions, thus opening implications in both environmental studies-for example, trucking the effects of heavy metal exposure on human or vegetable DNA molecules-and in vitro experiments for the evaluation of the effects of drugs on patient DNA.
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