期刊
BMC MOLECULAR AND CELL BIOLOGY
卷 23, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12860-022-00448-z
关键词
Ribosomal protein RPL5; Colon cancer; Proliferation; Migration; MAPK; ERK signaling pathway
类别
资金
- National Natural Science Foundation of China [81860444, 82060521]
- Natural Science Foundation of Shaanxi Province [2020JQ-801]
- Scientific Research Project of Shaanxi Provincial Department of Education [20JK0986]
- Innovation Team of Yan'an City-Innovation Team for Chronic Disease Prevention and Control [2018CXTD-03]
- Shaanxi Province Key Science and Technology innovation team [2020TD-039]
The high expression of RPL5 in colon cancer and its promotion of cell proliferation and migration through activating the MAPK/ERK pathway were found in this study. This finding may provide a new therapeutic target for cancers with dominant MAPK/ERK signaling.
Background Abnormal expression of ribosomal proteins has an important regulatory effect on the progression of cancer. RPL5 is involved in the progression of various malignancies, however, the role of RPL5 in colon cancer remains is still unclear. Methods Data from TCGA and GTEx databases were used to analyze the RPL5 expression in pan-cancer. The expression level of RPL5 in clinical colon cancer tissue samples and human colon cancer cell lines was detected by western blotting; siRNA targeting RPL5 was designed, and its interference efficiency was verified by western blotting and RT-qPCR; CCK8 assay, clone formation assay, cell cycle assay, and cell scratch assay were used to observe the effect of RPL5 on colon cancer cell proliferation and migration; the changes of proteins related to MAPK/ERK signaling pathway were also detected using western blotting. Results The expression level of RPL5 in colon cancer tissues and cell lines was significantly higher than that in adjacent tissues and NCM460 cells, respectively, and its expression level was higher in HCT116 cells and RKO cells. Knockdown of RPL5 significantly inhibited the proliferation and migration of HCT16 and RKO cells, and arrested the cell cycle in G0/G1 phase. Mechanistic studies revealed that the expression of p-MEK1/2, p-ERK, c-Myc were down-regulated, and the expression of FOXO3 was up-regulated after down-regulation of RPL5, ERK activator (TBHQ) could partially reverse the above-mentioned effects caused by siRPL5. Moreover, TBHQ could partially reverse the inhibitory effect of siRPL5 on the proliferation and migration of colon cancer cells. Collectively, RPL5 promoted colon cell proliferation and migration, at least in part, by activating the MAPK/ERK signaling pathway. Conclusion RPL5 promoted colon cell proliferation and migration, at least in part, by activating the MAPK/ERK signaling pathway, which may serve as a novel therapeutic target for cancers in which MAPK/ERK signaling is a dominant feature.
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