Design, synthesis and evaluation of novel 1,2,4-triazole derivatives as promising anticancer agents

Herein, we reported the synthesis of nineteen novel 1,2,4-triazole derivatives including 1,3-diphenyl-2-(1H-1,2,4-triazol-1-yl) propan-1-ones (7a-e), 1-(1,3-diphenylpropan-2-yl)-1H-1,2,4-triazole (8a-c) and 1,4-diphenyl-2-(1H-1,2,4-triazol-1-yl) butane-1,4-diones (10a-k). The structures of these derivatives were confirmed by spectroscopic techniques like IR, H-1-NMR, Mass spectroscopy and Elemental analysis. The cytotoxic activities of the synthesized compounds were evaluated against three human cancer cell lines including MCF-7, Hela and A549 using MTT assay. Compounds 7d, 7e, 10a and 10d showed a promising cytotoxic activity lower than 12 mu M against Hela cell line. The safety of these compounds was also, evaluated on MRC-5 as a normal cell line and relieved that most of the synthesized compounds have proper selectivity against normal and cytotoxic cancerous cell lines. Finally, molecular docking studies were also, done to understand the mechanism and binding modes of these derivatives in the binding pocket of aromatase enzyme as a possible target.

Automated summary

In this study, we synthesized nineteen novel 1,2,4-triazole derivatives and confirmed their structures using spectroscopic techniques. The cytotoxic activities of the synthesized compounds were evaluated against human cancer cell lines, and several compounds showed promising cytotoxic activity against the Hela cell line. The safety of these compounds was also assessed, and they exhibited proper selectivity against normal and cancerous cell lines. Additionally, molecular docking studies were conducted to understand the binding modes and mechanisms of these derivatives in the binding pocket of the aromatase enzyme as a potential target.