期刊
PLANT DIRECT
卷 6, 期 12, 页码 -出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/pld3.474
关键词
Arabidopsis; BPM; CRL3; development; E3 ligase; N-degron; stress; ubiquitin
资金
- USDA National Institute of Food and Agriculture (NIFA) [2019-67013-29160]
- Tafila Technical University, Jordan
Cullin-based RING E3 ligases using BTB/POZ-MATH proteins as substrate receptors play critical roles in plant development and abiotic stress tolerance. Researchers have developed a novel system to block the activity of this ligase by utilizing conserved protein-binding motifs, allowing for further study of its function in plants.
Cullin-based RING E3 ligases that use BTB/POZ-MATH (BPM) proteins as substrate receptors have been established over the last decade as critical regulators in plant development and abiotic stress tolerance. As such they affect general aspects of shoot and root development, flowering time, embryo development, and different abiotic stress responses, such as heat, drought and salt stress. To generate tools that can help to understand the role of CRL3(BPM) E3 ligases in plants, we developed a novel system using two conserved protein-binding motifs from BPM substrates to transiently block CRL3(BPM) activity. The work investigates in vitro and in planta this novel approach, and shows that it can affect stress tolerance in plants as well as developmental aspects. It thereby can serve as a new tool for studying this E3 ligase in plants.
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