Determination of Cordycepin Using a Stability-Indicating Greener HPTLC Method

A wide range of analytical techniques have been reported to determine cordycepin (CDN) in various sample matrices. Nevertheless, greener analytical approaches for CDN estimation are scarce in the literature. As a result, this study was designed to develop and validate a stability-indicating greener high-performance thin-layer chromatography (HPTLC) technique for CDN determination in a laboratory-developed formulation. The greener eluent system for CDN detection was ethanol-water (75:25 v/v). At a wavelength of 262 nm, CDN was measured. The greenness scale of the proposed analytical technology was derived using the Analytical GREENness (AGREE) approach. The proposed stability-indicating HPTLC assay was linear for CDN analysis in the 50-1000 ng/band range with a determination coefficient of 0.9978. The proposed analytical technique for CDN analysis was simple, rapid, accurate, precise, robust, selective, stability-indicating, and greener. The AGREE score for the proposed stability-indicating HPTLC technique was calculated to be 0.79 using the AGREE calculator. The current protocol was able to detect CDN degradation products under various stress conditions, indicating its stability-indication characteristics and selectivity. The AGREE quantitative score indicated that the stability-indicating current protocol had outstanding greener characteristics. The amount of CDN in the laboratory-developed formulation was determined to be 98.84%, indicating the suitability of the current protocol in the assay of CDN in the formulations. These results suggested that CDN in a laboratory-developed formulation may be regularly determined using the stability-indicating greener HPTLC strategy.

Automated summary

In this study, a stability-indicating greener high-performance thin-layer chromatography (HPTLC) technique was developed to determine cordycepin (CDN) in a laboratory-developed formulation. The proposed analytical technique showed accurate, rapid, simple, and stable CDN determination, with an AGREE score of 0.79 indicating outstanding greener characteristics. The method was suitable for the assay of CDN in the formulations.