期刊
JOURNAL OF SEPARATION SCIENCE
卷 39, 期 23, 页码 4557-4567出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600718
关键词
Chromatographic Fingerprints; High-performance liquid chromatography; Quality evaluation; Quantitative analysis; Traditional Chinese medicine
资金
- Program for Liaoning Innovative Research Team in University [LT 20121018]
- Project of Liaoning Distinguished Professor
- National Undergraduate Training Programs for Innovation and Entrepreneurship [201510163000014]
In this study, we aimed to establish a comprehensive and practical quality evaluation system for Shenmaidihuang pills. A simple and reliable high-performance liquid chromatography coupled with photodiode array detectionmethod was developed both for fingerprint analysis and quantitative determination. In fingerprint analysis, relative retention time and relative peak area were used to identify the common peaks in 18 samples for investigation. Twenty one peaks were selected as the common peaks to evaluate the similarities of 18 Shenmaidihuang pills samples with different manufacture dates. Furthermore, similarity analysis was applied to evaluate the similarity of samples. Hierarchical cluster analysis and principal component analysis were also performed to evaluate the variation of Shenmaidihuang pills. In quantitative analysis, linear regressions, injection precisions, recovery, repeatability and sample stability were all tested and good results were obtained to simultaneously determine the seven identified compounds, namely, 5-hydroxymethylfurfural, morroniside, loganin, paeonol, paeoniflorin, psoralen, isopsoralen in Shenmaidihuang pills. The contents of some analytes in different batches of samples indicated significant difference, especially for 5-hydroxymethylfurfural. So, it was concluded that the chromatographic finger print method obtained by high-performance liquid chromatography coupled with photodiode array detection associated with multiple compounds determination is a powerful and meaningful tool to comprehensively conduct the quality control of Shenmaidihuang pills.
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