期刊
JOURNAL OF SEPARATION SCIENCE
卷 39, 期 12, 页码 2365-2373出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201501406
关键词
Boronate affinity; Cotton fiber; Nucleosides; Pipette-tip solid-phase extraction; Polyhedral oligomeric silsesquioxanes
资金
- National Natural Science Foundation of China [21275115, 21475104, 21575114]
Various cotton fiber based boronate-affinity adsorbents are recently developed for the sample pretreatment of cis-diol-containing biomolecules, butmost do not have efficient capacity due to limited binding sites on the surface of cotton fibers. To increase the density of boronate groups on the surface of cotton fiber, polyhedral oligomeric silsesquioxanes were used to modify cotton fiber to provide plentiful reactive sites for subsequent functionalization with 4-formylphenylboronic acid. The new adsorbent showed special recognition ability towards cis-diols and high adsorption capacity (175 mu g/g for catechol, 250 mu g/g for dopamine, 400 mu g/g for adenosine). The in-pipette-tip solid-phase extraction was investigated under different conditions, including pH and ionic strength of solution, adsorbent amount, pipette times, washing solvent, and elution solvent. The in-pipette-tip solid-phase extraction coupled with high-performance liquid chromatography was used to analyze four nucleosides in urine samples. Under the optimal extraction conditions, the detection limits were determined to be between 5.1 and 6.1 ng/mL (S/N = 3), and the linearity ranged from 20 to 500 ng/mL for these analytes. The accuracy of the analytical method was examined by studying the relative recoveries of analytes in real urine samples with recoveries varying from 83 to 104% (RSD = 3.9-10.2%, n = 3).
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