期刊
JOURNAL OF SEPARATION SCIENCE
卷 39, 期 12, 页码 2374-2379出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600004
关键词
Antioxidant; Counter-current chromatography; Isomers; Wine grapes
资金
- National Natural Sciences Foundation of China [31260402, 31460411, 21362028]
- Fundamental Research Funds for the Central Universities [GK201503069, GK201603095]
- Agricultural science and technology innovation and research [2016NY-184, 2016NY-195]
Resveratrol dimers belong to a group of compounds called stilbenes, which along with proanthocyanidins, anthocyanins, catechins, and flavonols are natural phenolic compounds found in grapes and red wine. Stilbenes have a variety of structural isomers, all of which exhibit various biological properties. Counter-current chromatography with a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (2:5:4:5, v/v/v/v) was applied to isolate and purify stilbene from the stems of wine grape. Two isomers of resveratrol dimers trans-epsilon-viniferin and trans-delta-viniferin were obtained from the crude sample in a one-step separation, with purities of 93.2 and 97.5%, respectively, as determined by high-performance liquid chromatography. The structures of these two compounds were identified by H-1 and C-13 NMR spectroscopy. In addition, their antioxidant activities were assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activities of trans-delta-viniferin were higher than that of trans-epsilon-viniferin in this model. This work demonstrated that counter-current chromatography is a powerful and effective method for the isolation and purification of polyphenols from wine grape. Additionally, the DPPH radical assay showed that the isolated component trans-delta-viniferin exhibited stronger antioxidant activities than trans-g-viniferin and a little bit weaker than vitamin E at the same concentration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据