期刊
BIOSENSORS-BASEL
卷 12, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/bios12111014
关键词
surface plasmon coupled emission; cell imaging; variable-angle nanoplasmonic fluorescence microscopy; alloy nanoshuttle; fluorescence enhancement
资金
- National Natural Science Foundation of China
- Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi
- [21874110]
- [2274137]
- [21804098]
- [21974117]
- [Education-SCAI2003]
- [2021L402]
Surface plasmon-coupled emission (SPCE) is a signal enhancement technology that has shown excellent performance in bioimaging. By controlling the excitation angles, the imaging depth can be selectively modulated in a variable-angle nanoplasmonic fluorescence microscopy (VANFM) system based on SPCE. The introduction of Au-Ag alloy nanoshuttles improves imaging performance by mediating the plasmonic properties, leading to better imaging brightness, signal-to-background ratio, and axial resolution.
Surface plasmon-coupled emission (SPCE), a novel signal enhancement technology generated by the interactions between surface plasmons and excited fluorophores in close vicinity to metallic film, has shown excellent performance in bioimaging. Variable-angle nanoplasmonic fluorescence microscopy (VANFM), based on an SPCE imaging system, can selectively modulate the imaging depth by controlling the excitation angles. In order to further improve the imaging performance, Au-Ag alloy nanoshuttles were introduced into an Au substrate to mediate the plasmonic properties. Benefiting from the strong localized plasmon effect of the modified SPCE chip, better imaging brightness, signal-to-background ratio and axial resolution for imaging of the cell membrane region were obtained, which fully displays the imaging advantages of SPCE system. Meanwhile, the imaging signal obtained from the critical angle excitation mode was also amplified, which helps to acquire a more visible image of the cell both from near- and far-field in order to comprehensively investigate the cellular interactions.
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