期刊
BIOSENSORS-BASEL
卷 12, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/bios12121146
关键词
porcine epidemic diarrhea virus (PEDV); gold-nanorod probe (GNR probe); dark field microscopy (DFM); low detection limit; on-site detection
资金
- National Natural Science Foundation of China [31870989]
- Shanghai Science and Technology Innovation Action Plan in 2022 [22N31900800]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) - Hospital Research Foundation Group
- Hospital Research Foundation Group
In this study, a method combining gold nanorod probes and dark field microscopy was proposed for rapid and quantitative detection of porcine epidemic diarrhea virus. The method showed strong specificity, good repeatability, and low detection limit, making it applicable for rapid on-site detection of pathogens.
Porcine epidemic diarrhea virus (PEDV), a coronavirus that causes highly infectious intestinal diarrhea in piglets, has led to severe economic losses worldwide. Rapid diagnosis and timely supervision are significant in the prophylaxis of PEDV. Herein, we proposed a gold-nanorod (GNR) probe-assisted counting method using dark field microscopy (DFM). The antibody-functionalized silicon chips were prepared to capture PEDV to form sandwich structures with GNR probes for imaging under DFM. Results show that our DFM-based assay for PEDV has a sensitivity of 23.80 copies/mu L for simulated real samples, which is very close to that of qPCR in this study. This method of GNR probes combined with DFM for quantitative detection of PEDV not only has strong specificity, good repeatability, and a low detection limit, but it also can be implemented for rapid on-site detection of the pathogens.
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