期刊
BIOSENSORS-BASEL
卷 13, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/bios13020218
关键词
fluorescent protein-based biosensors; calcium sensing; ultrafast molecular spectroscopy; femtosecond stimulated Raman spectroscopy; protein rational design
Fluorescent proteins (FPs) are important tools for noninvasive bioimaging and sensing. REX-GECO1, a red-green emission and excitation ratiometric FP-based biosensor, shows a high dynamic range in measuring cellular calcium concentrations. The potential energy surfaces of these biosensors are revealed using electronic and spectroscopic techniques, uncovering details of their behavior. These findings can inform the design of biosensors with improved efficiency for calcium imaging.
Fluorescent proteins (FPs) are indispensable tools for noninvasive bioimaging and sensing. Measuring the free cellular calcium (Ca2+) concentrations in vivo with genetically encodable FPs can be a relatively direct measure of neuronal activity due to the complex signaling role of these ions. REX-GECO1 is a recently developed red-green emission and excitation ratiometric FP-based biosensor that achieves a high dynamic range due to differences in the chromophore response to light excitation with and without calcium ions. Using steady-state electronic measurements (UV/Visible absorption and emission), along with time-resolved spectroscopic techniques including femtosecond transient absorption (fs-TA) and femtosecond stimulated Raman spectroscopy (FSRS), the potential energy surfaces of these unique biosensors are unveiled with vivid details. The ground-state structural characterization of the Ca2+-free biosensor via FSRS reveals a more spacious protein pocket that allows the chromophore to efficiently twist and reach a dark state. In contrast, the more compressed cavity within the Ca2+-bound biosensor results in a more heterogeneous distribution of chromophore populations that results in multi-step excited state proton transfer (ESPT) pathways on the sub-140 fs, 600 fs, and 3 ps timescales. These results enable rational design strategies to enlarge the spectral separation between the protonated/deprotonated forms and the Stokes shift leading to a larger dynamic range and potentially higher fluorescence quantum yield, which should be broadly applicable to the calcium imaging and biosensor communities.
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